Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRX1801650

SRA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

19/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Illumina HiSeq 2500 bulk RNA-seq of Saccharomyces cerevisiae generates ~20 million reads (~1 Gb) with moderate quality (87.7% Q20, 73% Q30) from an older instrument, enabling yeast-transcriptome profiling despite moderate data quality. The model-organism context supports robust analysis despite suboptimal sequencing metrics. Researchers seeking yeast transcriptomics will find this useful.

Data type / assay
bulk-RNA-seq
Organism
Saccharomyces cerevisiae
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
2 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 1002467150 reported
total reads 20049343 reported
n content pct 0.225 measured
pct q20 bases 87.7 measured
pct q30 bases 73 measured
gc content pct 49.9 measured
mean read length 50 measured
mean base quality 30.6 measured
adapter content pct 78.25 measured
duplication rate pct 69.04 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 19/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 73 measured ×1 15%
mean base quality 30.6 measured ×0.6 43%
adapter content pct 78.25 measured ×0.4 0%
duplication rate pct 69.04 measured ×0.4 13%
QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0