Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRX191169

ENA first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

62/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Tetraodon nigroviridis (pufferfish) bulk RNA-Seq on HiSeq 2000 spans 11.4 Gb with 75 million reads and perfect Q30 (100%), providing exceptional-quality transcriptome data for this compact-genome vertebrate model. The clean short reads enable precise isoform discovery and transcript quantification. Vertebrate genomics and developmental biology can leverage this pristine pufferfish RNA-seq.

Data type / assay
bulk-RNA-seq
Organism
Tetraodon nigroviridis
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 11433586224 reported
total reads 75220962 reported
n content pct 0.002 measured
pct q20 bases 100 measured
pct q30 bases 100 measured
gc content pct 53.8 measured
mean read length 76 measured
mean base quality 30 measured
adapter content pct 19.09 measured
duplication rate pct 44.09 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 62/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%
mean base quality 30 measured ×0.6 33%
adapter content pct 19.09 measured ×0.4 5%
duplication rate pct 44.09 measured ×0.4 69%
QC cost 59 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0