Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

SRX287395

ENA first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

89/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Glossina morsitans (tsetse fly) RNA-Seq on HiSeq 1000 spans 3.1 Gb with 42 million reads and moderate quality (93.3% Q20, 85.5% Q30), providing transcriptome coverage for this vector of sleeping sickness. The dataset enables gene-expression profiling related to parasite transmission, reproduction, and immunity. Vector-biology and trypanosome-interaction studies can use this tsetse RNA-seq to understand molecular mechanisms of vector competence.

Data type / assay
bulk-RNA-seq
Organism
Glossina morsitans morsitans
Instrument
Illumina HiSeq 1000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 3132077158 reported
total reads 42325367 reported
n content pct 0.105 measured
pct q20 bases 93.3 measured
pct q30 bases 85.5 measured
gc content pct 38.6 measured
mean read length 74 measured
mean base quality 34.5 measured
adapter content pct 0.75 measured
duplication rate pct 35.11 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 89/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.5 measured ×1 78%
mean base quality 34.5 measured ×0.6 100%
adapter content pct 0.75 measured ×0.4 100%
duplication rate pct 35.11 measured ×0.4 89%
QC cost 55 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0