Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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SRX2894153

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This third Oncorhynchus mykiss RNA-Seq on Genome Analyzer IIx spans 8.7 Gb with 43.3 million reads and moderate quality (90% Q20, 82.7% Q30), providing a third transcriptome sample for rainbow-trout expression studies. The three O. mykiss datasets enable multi-condition or developmental-time-course comparisons. Aquaculture and salmonid stress-response studies can integrate all three trout RNA-seq samples.

Data type / assay
bulk-RNA-seq
Organism
Oncorhynchus mykiss
Instrument
Illumina Genome Analyzer IIx
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 8664377000 reported
total reads 43321885 reported
n content pct 0.042 measured
pct q20 bases 90 measured
pct q30 bases 82.7 measured
gc content pct 51 measured
mean read length 100 measured
mean base quality 33.2 measured
adapter content pct 0.26 measured
duplication rate pct 49.7 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.7 measured ×1 64%
mean base quality 33.2 measured ×0.6 87%
adapter content pct 0.26 measured ×0.4 100%
duplication rate pct 49.7 measured ×0.4 56%
QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0