SRX326765

Whole-genome shotgun sequencing of the Asian longhorned beetle (Anoplophora glabripennis) on Illumina HiSeq 2000 — short 101 bp reads at roughly 14.6 Gb total. In QC terms this dataset earns a failing grade, driven overwhelmingly by a duplication rate of 39.36%, which scored just 2/100: such heavy read redundancy means a large fraction of the ~72M reads are non-independent PCR or optical duplicates, inflating apparent depth while contributing little real coverage and biasing variant calling and any coverage-based analysis like assembly or CNV detection. Per-base quality is mediocre but not disqualifying — 81.4% of bases at Q30 (57/100) is acceptable for variant work but signals some error accumulation, while low adapter (2.06%) and negligible N content are genuine strengths. Most core quality metrics here were actually measured, so the duplication and quality readings are reliable; however, the headline totals (read and base counts) are only reported rather than independently verified, and the very low evidence_strength flag means the overall grade should be treated as provisional pending a fuller measured pass. For reuse, you can likely salvage usable data by aggressive duplicate removal, but budget for the effective coverage loss that implies.