SRX6649471

ENA first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

78/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon sequencing, Solanum. Grade C; adapter content (10.37%, scored 33/100) is elevated and duplication (73.68%) very high—both expected but indicating suboptimal PCR efficiency. Suitable for presence/absence profiling but abundance quantitation unreliable.

Data type / assay

amplicon

Organism

Solanum

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 74698800 reported

total reads 497992 reported

n content pct 0 measured

pct q20 bases 94 measured

pct q30 bases 90.4 measured

gc content pct 40.9 measured

mean read length 150 measured

mean base quality 33.8 measured

adapter content pct 10.37 measured

duplication rate pct 73.68 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 78/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.4 measured ×1 100%

adapter content pct 10.37 measured ×0.5 33%

QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0