SRX6744194

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

96/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

E. coli short-read WGS with exceptional sequencing depth. Mean coverage (134.8x, extrapolated) strongly supports assembly and variant discovery, but 16.6% duplication rate (measured) indicates PCR bias that warrants caution for copy-number and structural variant analysis. Evidence strength 0.61 is provisional; deep measured pass pending.

Data type / assay

WGS

Organism

Escherichia coli

Instrument

HiSeq X Ten

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 620150599 reported

total reads 1860203 reported

mean coverage 134.8 extrapolated

n content pct 0 measured

pct q20 bases 94.8 measured

pct q30 bases 90.3 measured

gc content pct 49.3 measured

mean read length 233.6 measured

mean base quality 36 measured

adapter content pct 0 measured

duplication rate pct 16.6 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 96/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

mean coverage 134.8 extrapolated ×1.2 100%

pct q30 bases 90.3 measured ×1 100%

duplication rate pct 16.6 measured ×0.5 73%

adapter content pct 0 measured ×0.4 100%

QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0