SRX735409

ENA first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk miRNA-seq data (Homo sapiens), grade B but severely limited by 88.27% duplication, which is catastrophic for miRNA profiling. This extreme duplication renders individual miRNA abundance estimates unreliable due to PCR cloning bias. Superior base quality (Q30 97.1%) cannot compensate; reuse is not advised without deduplication and careful abundance validation.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2716004750 reported

total reads 54320095 reported

n content pct 1.412 measured

pct q20 bases 99.1 measured

pct q30 bases 97.1 measured

gc content pct 55.8 measured

mean read length 50 measured

mean base quality 37.9 measured

adapter content pct 0 measured

duplication rate pct 88.27 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.1 measured ×1 100%

mean base quality 37.9 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 88.27 measured ×0.4 0%

QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0