SRX796311

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina RNA-seq from Prunus persica with marginal QC (85/100, B grade). Suboptimal Q30 (83%, scored 65/100, weight 1.0) significantly compromises expression quantification accuracy. Acceptable only for preliminary exploratory work; recommend fresh sequencing for rigorous expression studies.

Data type / assay

bulk-RNA-seq

Organism

Prunus persica

Instrument

Illumina Genome Analyzer IIx

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2391464927 reported

total reads 16052080 reported

n content pct 0.013 measured

pct q20 bases 96.1 measured

pct q30 bases 83 measured

gc content pct 45.5 measured

mean read length 72.4 measured

mean base quality 34.5 measured

adapter content pct 0 measured

duplication rate pct 24.55 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83 measured ×1 65%

mean base quality 34.5 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 24.55 measured ×0.4 100%

QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0