Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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SRX8089276

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

77/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Homo sapiens bulk-RNA-seq scored C (77/100) with notably lower base quality than peer datasets (82.2% Q30, 32.3 mean), reflecting either run degradation or suboptimal library quality (longer 135.8 nt reads may amplify downstream noise). While checksum and file availability are confirmed, base-call accuracy is a bottleneck; recommend validation before sensitive quantification work.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 1263198214 reported
total reads 9324151 reported
n content pct 0.027 measured
pct q20 bases 89.6 measured
pct q30 bases 82.2 measured
gc content pct 51 measured
mean read length 135.8 measured
mean base quality 32.3 measured
adapter content pct 0.23 measured
duplication rate pct 25.41 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 77/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.2 measured ×1 61%
mean base quality 32.3 measured ×0.6 72%
adapter content pct 0.23 measured ×0.4 100%
duplication rate pct 25.41 measured ×0.4 100%
QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0