SRX8089277

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina RNA-seq from human tissue with low QC (74/100, C grade). Suboptimal Q30 (81.5%, scored 57/100, weight 1.0) and reduced base quality (Q-score: 32.1, scored 68/100, weight 0.6) together significantly limit expression quantification confidence. Acceptable only for preliminary surveys; recommend fresh sequencing for rigorous expression studies.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 2389795881 reported

total reads 17436078 reported

n content pct 0.083 measured

pct q20 bases 89.2 measured

pct q30 bases 81.5 measured

gc content pct 53.2 measured

mean read length 137.3 measured

mean base quality 32.1 measured

adapter content pct 0.26 measured

duplication rate pct 27.06 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.5 measured ×1 57%

mean base quality 32.1 measured ×0.6 68%

adapter content pct 0.26 measured ×0.4 100%

duplication rate pct 27.06 measured ×0.4 100%

QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0