SRX8089280

SRA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Homo sapiens bulk RNA-seq with moderate quality and elevated PCR amplification. Q30 bases (84.2%, measured) fall below the 85–90% threshold, and elevated duplication (35.29%, measured) suggests low input RNA or over-cycling. Usable for expression in well-covered genes; risk of noise in low-abundance transcripts.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1908376184 reported

total reads 13583713 reported

n content pct 0.01 measured

pct q20 bases 91 measured

pct q30 bases 84.2 measured

gc content pct 48.5 measured

mean read length 140.6 measured

mean base quality 32.6 measured

adapter content pct 0.14 measured

duplication rate pct 35.29 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84.2 measured ×1 71%

mean base quality 32.6 measured ×0.6 77%

adapter content pct 0.14 measured ×0.4 100%

duplication rate pct 35.29 measured ×0.4 88%

QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0