Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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SRX871300

SRA first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

89/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bothrioplana semperi bulk-RNA-seq achieves grade B (89/100) with high-quality base composition (96.5% Q30) but is compromised by high read duplication (51.46%, scoring 52/100) and elevated adapter contamination (4.92%, scoring 84/100), likely indicating PCR-driven amplification or library preparation issues. Consider this dataset suitable mainly for coarse-grained analyses; transcript abundance estimates may be biased.

Data type / assay
bulk-RNA-seq
Organism
Bothrioplana semperi
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 7205297176 reported
total reads 35669788 reported
n content pct 0.002 measured
pct q20 bases 99 measured
pct q30 bases 96.5 measured
gc content pct 51.1 measured
mean read length 101 measured
mean base quality 36.8 measured
adapter content pct 4.92 measured
duplication rate pct 51.46 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 89/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.5 measured ×1 100%
mean base quality 36.8 measured ×0.6 100%
adapter content pct 4.92 measured ×0.4 84%
duplication rate pct 51.46 measured ×0.4 52%
QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0