SRX873912

This is bulk RNA-seq from the Asian longhorned beetle (Anoplophora glabripennis) on an Illumina HiSeq 2000, and on QC terms it earns a solid A (91/100) as a fully measured library you can confidently reuse. The grade is anchored by excellent base-call quality — 89.9% of bases at Q30 and a mean base quality of 35.4, with negligible adapter (0.01%) and N content — meaning reads are clean and trustworthy for alignment and expression quantification with minimal trimming. The one real drag is a duplication rate of 52.45%, which scored only 50/100 and is the single metric pulling the grade down; for RNA-seq some duplication is expected from highly expressed transcripts, but a level this high can also signal limited library complexity or PCR over-amplification, so deduplication choices may bias quantification and you should check complexity before relying on lowly-expressed genes. Crucially, all quality-driving metrics here are directly measured (evidence_strength=1), so this reading is firm rather than provisional, though the total_reads/total_bases counts are reported rather than independently verified.