Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

SRX873913

SRA

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

89/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a bulk RNA-seq library from the Asian longhorned beetle (Anoplophora glabripennis) sequenced on an Illumina HiSeq 2000, and it earns a solid B (89/100) with all scored metrics actually measured, so the reading is firm rather than provisional. The grade is propped up by excellent base-call quality — a mean Q of 35.1 and 89.3% of bases at Q30, with negligible adapter (0.01%) and N content — meaning per-base accuracy is high and little trimming is needed before alignment. The single factor holding it back is a high duplication rate of 54.97% (scored 44/100), which for RNA-seq partly reflects genuinely abundant transcripts but also signals possible PCR/optical redundancy that can bias expression estimates and inflate apparent depth; you should deduplicate cautiously and treat effective library complexity as lower than the raw 33M reads suggest. Overall the data are trustworthy for differential-expression and assembly reuse, but plan for the duplication when judging quantitative sensitivity.

Data type / assay
bulk-RNA-seq
Organism
Anoplophora glabripennis
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 6719579490 reported
total reads 33265245 reported
n content pct 0.002 measured
pct q20 bases 95.8 measured
pct q30 bases 89.3 measured
gc content pct 45.8 measured
mean read length 101 measured
mean base quality 35.1 measured
adapter content pct 0.01 measured
duplication rate pct 54.97 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 89/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 89.3 measured ×1 97%
mean base quality 35.1 measured ×0.6 100%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 54.97 measured ×0.4 45%
QC cost 10 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 84