Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

Publications 1.3k Datasets 392.5k Experiments 24

System / cell line: kinase ✕ Assay: activity ✕ Clear

Finding the needle in the haystack: why high-throughput screening is good for your health.

PMID 12100740 · PMC138735 · Breast cancer research : BCR · 2002 · 8 claims · 8 setups

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HTS is essential for finding lead compounds, especially for novel targets whose active-site structure is unknown

raf-1 mitotic mutant tumor biochemical cell-based conformational
CHEK2 mutations affecting kinase activity together with mutations in TP53 indicate a functional pathway associated with resistance to epirubicin in primary breast cancer.

PMID 18725978 · PMC2518116 · PloS one · 2008 · 7 claims · 6 setups

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TP53 mutations, especially those affecting the L2/L3 DNA-binding domains, are associated with resistance (progressive disease) to epirubicin therapy

snap-frozen tumor u-2-os patients pcr bisulphite loss
Sequence-non-specific effects of RNA interference triggers and microRNA regulators.

PMID 19843612 · PMC2800214 · Nucleic acids research · 2010 · 8 claims · 7 setups

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Reagents of RNAi and miRNA technologies often trigger unintended sequence-non-specific immune responses in addition to intended sequence-specific silencing

recombinant t98g hek293 hela kinase pkr microarray
Raf-1 activation disrupts its binding to keratins during cell stress.

PMID 15314064 · PMC2172217 · The Journal of cell biology · 2004 · 8 claims · 8 setups

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Raf-1 kinase associates directly with keratin K8 (not K18), independent of Raf kinase activity and independent of Ras-Raf interaction

ht29 transgenic bhk-21 bhk co-immunoprecipitation co-transfection oxidative
Structural insights into the inhibited states of the Mer receptor tyrosine kinase.

PMID 19028587 · PMC2686088 · Journal of structural biology · 2009 · 8 claims · 8 setups

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Nucleotide-bound (ADP and ANP/AMP-PNP) Mer kinase domain adopts an autoinhibited DFG-Asp-in/αC-Glu-out conformation with an activation-loop residue inserted into the active site

recombinant phosphorylated mer x-ray positional differential
A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation.

PMID 36149401 · PMC9576273 · eLife · 2022 · 8 claims · 8 setups

Has reproduction · 50

A C-terminal patch of the LRRK2 Armadillo domain (residues ~350–550, 'site #1') binds non-phosphorylated Rab29, Rab8A, and Rab10 with low-micromolar affinity

recombinant lrrk2 hela hek293t microscale confocal immunoblotting
Differential Hsp90-dependent gene expression is strain-specific and common among yeast strains.

PMID 37138775 · PMC10149407 · iScience · 2023 · 8 claims · 7 setups

Has reproduction · 100

Hsp90-dependent gene expression varies among different yeast strains

diverse same yeast genomes rna-seq v-src growth
Patterns of somatic mutation in human cancer genomes.

PMID 17344846 · PMC2712719 · Nature · 2007 · 8 claims · 5 setups

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Systematic resequencing of a large gene family (protein kinases) across diverse cancers reveals a larger repertoire of cancer genes than previously anticipated

cancers additional computational combined targeted follow-up statistical
Direct resequencing of the complete ERBB2 coding sequence reveals an absence of activating mutations in ERBB2 amplified breast cancer.

PMID 18418848 · PMC6668724 · Genes, chromosomes & cancer · 2008 · 6 claims · 5 setups

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Emulsion PCR combined with picotiter plate pyrosequencing (454 sequencing) enables high-resolution, high-throughput detection of low-frequency sequence variants among the many individual copies of an amplified gene.

erbb2-amplified erbb2-positive plasmid cos-7 emulsion serial immune-complex
Reconstruction of pathways associated with amino acid metabolism in human mitochondria.

PMID 18267298 · PMC5054205 · Genomics, proteomics & bioinformatics · 2007 · 8 claims · 5 setups

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Out of 20 amino acids, the metabolic pathways of 17 utilize mitochondrial enzymes, and dysfunction of these enzymes causes over 40 known human mitochondrial diseases/disorders

mitoproteome orthologous liver mitochondrial sequence-based computational literature
Centromere-associated protein-E is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single chromosome loss.

PMID 12925705 · PMC2173788 · The Journal of cell biology · 2003 · 7 claims · 8 setups

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CENP-E-null cells cannot sustain a mitotic checkpoint arrest in response to one or a few unattached kinetochores, causing cells to enter anaphase with missegregating chromosomes and become aneuploid

embryonic mefs hepatocytes bubr1 real-time quantitative immunofluorescence
Protein microarray technology.

PMID 17126887 · PMC1828913 · Mechanisms of ageing and development · 2007 · 8 claims · 8 setups

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Protein microarrays enable high-throughput characterization of protein biochemical activities across an entire proteome in a single experiment

yeast sh2 protein-protein protein-dna chromatin
Physiology engages with functional genomics - at last.

PMID 16086845 · PMC1273626 · Genome biology · 2005 · 8 claims · 8 setups

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Large-scale QTL phenotyping in rat strains reveals that most hypertension-related traits are sexually dimorphic

wild-type caenorhabditis kidney pcr qtl enu copas
Defactinib inhibits PYK2 phosphorylation of IRF5 and reduces intestinal inflammation.

PMID 34795257 · PMC8602323 · Nature communications · 2021 · 8 claims · 12 setups

Has reproduction · 67

PYK2 was identified as a putative IRF5 kinase via a kinase inhibitor library screen in macrophages

raw264.7 hektlr4 hoxb8-derived colon kinase co-immunoprecipitation co-ip
Tyrosine phosphorylation inhibits PKM2 to promote the Warburg effect and tumor growth.

PMID 19920251 · PMC2812789 · Science signaling · 2009 · 7 claims · 8 setups

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Oncogenic FGFR1 directly phosphorylates PKM2 at tyrosine 105 (Y105), inhibiting its enzymatic activity

293t kg-1a recombinant h1299 ms-based co-transfection enzymatic
Systematic analysis of human kinase genes: a large number of genes and alternative splicing events result in functional and structural diversity.

PMID 16351747 · PMC1866387 · BMC bioinformatics · 2005 · 8 claims · 7 setups

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Systematic in silico search identified 5 novel human kinase genes (on chromosomes 1, 11, 13, 15, 16) and 1 pseudogene (chromosome X) absent from KinBase

genome genomic kinase psi-blast comparative genscan
Proteomics and genomics: perspectives on drug and target discovery.

PMID 18302945 · PMC2386992 · Current opinion in chemical biology · 2008 · 8 claims · 8 setups

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Protein kinases account for approximately 25% of research and development projects in biotechnology and pharma

proteins blood carbohydrate medicinal x-ray microarrays glycan
RSK1 is an exploitable dependency in myeloproliferative neoplasms and secondary acute myeloid leukemia.

PMID 39820365 · PMC11739599 · Nature communications · 2025 · 8 claims · 8 setups

Has reproduction · 73

RSK1 is a conserved, exploitable therapeutic dependency across MPN and secondary AML

sorted mpn cd34+ aml bulk targeted mass
A Korean family with Arg1448Cys mutation of SCN4A channel causing paramyotonia congenita: electrophysiologic, histopathologic, and molecular genetic studies.

PMID 12483017 · PMC3054970 · Journal of Korean medical science · 2002 · 7 claims · 5 setups

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A missense mutation (Arg1448Cys, R1448C) in SCN4A causes paramyotonia congenita in this Korean family

skeletal abductor genomic proband needle short muscle
Molecular genetic analysis of an endotoxin nonresponder mutant cell line: a point mutation in a conserved region of MD-2 abolishes endotoxin-induced signaling.

PMID 11435474 · PMC2193443 · The Journal of experimental medicine · 2001 · 8 claims · 7 setups

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MD-2 is a required component of the LPS signaling complex; a point mutation in MD-2 abolishes LPS-induced signaling

cho hek western nf-kb il-6

Experiments

Finding the needle in the haystack: why high-throughput screening is good for your health.

CHEK2 mutations affecting kinase activity together with mutations in TP53 indicate a functional pathway associated with resistance to epirubicin in primary breast cancer.

Sequence-non-specific effects of RNA interference triggers and microRNA regulators.

Raf-1 activation disrupts its binding to keratins during cell stress.

Structural insights into the inhibited states of the Mer receptor tyrosine kinase.

A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation.

Differential Hsp90-dependent gene expression is strain-specific and common among yeast strains.

Patterns of somatic mutation in human cancer genomes.

Direct resequencing of the complete ERBB2 coding sequence reveals an absence of activating mutations in ERBB2 amplified breast cancer.

Reconstruction of pathways associated with amino acid metabolism in human mitochondria.

Centromere-associated protein-E is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single chromosome loss.

Protein microarray technology.

Physiology engages with functional genomics - at last.

Defactinib inhibits PYK2 phosphorylation of IRF5 and reduces intestinal inflammation.

Tyrosine phosphorylation inhibits PKM2 to promote the Warburg effect and tumor growth.

Systematic analysis of human kinase genes: a large number of genes and alternative splicing events result in functional and structural diversity.

Proteomics and genomics: perspectives on drug and target discovery.

RSK1 is an exploitable dependency in myeloproliferative neoplasms and secondary acute myeloid leukemia.

A Korean family with Arg1448Cys mutation of SCN4A channel causing paramyotonia congenita: electrophysiologic, histopathologic, and molecular genetic studies.

Molecular genetic analysis of an endotoxin nonresponder mutant cell line: a point mutation in a conserved region of MD-2 abolishes endotoxin-induced signaling.