Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

Publications 1.3k Datasets 392.5k Experiments 40

System / cell line: sections ✕ Assay: blot ✕ Clear

The use of laser capture microscopy in proteomics research--a review.

PMID 15502248 · PMC3839334 · Disease markers · 2004 · 8 claims · 8 setups

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LCM allows purification of specific, histologically defined cell populations from heterogeneous tissue for downstream proteomic analysis

breast prostate esophageal head maldi-tof automated two-dimensional
A missense mutation (Q279R) in the fumarylacetoacetate hydrolase gene, responsible for hereditary tyrosinemia, acts as a splicing mutation.

PMID 11476670 · PMC35353 · BMC genetics · 2001 · 8 claims · 7 setups

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The Q279R missense mutation acts as a splicing mutation in vivo, causing skipping of exon 9 (alone or with exon 8) rather than simply altering the encoded amino acid.

microdissected resected non-tumor liver pcr-rflp immunohistochemistry western
Molecular dissection of ALS-associated toxicity of SOD1 in transgenic mice using an exon-fusion approach.

PMID 18424447 · PMC2465800 · Human molecular genetics · 2008 · 6 claims · 8 setups

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Nonsense-mediated mRNA decay (NMD) degrades mutant SOD1 mRNA carrying a PTC in non-terminal exons (1-4), explaining why ALS-associated PTC mutations are found only in exon 5

transgenic nih hsod1 lumbar southern northern rt-pcr
Breast cancer proteomics reveals correlation between estrogen receptor status and differential phosphorylation of PGRMC1.

PMID 18922159 · PMC2614521 · Breast cancer research : BCR · 2008 · 8 claims · 5 setups

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PGRMC1 protein spots are differentially abundant between ER-negative and ER-positive breast tumors, with two of three spots more abundant in ER-negative tumors

sections pooled mcf7 paraffin-embedded 2d-page shrimp viability
Expression of p73, a novel protein related to the p53 tumour suppressor p53, and apoptosis in cholangiocellular carcinoma of the liver.

PMID 10362118 · PMC2363034 · British journal of cancer · 1999 · 7 claims · 6 setups

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p73 protein is expressed (nuclear immunostaining) in a substantial subset of cholangiocellular carcinomas

cholangiocellular ccc lysates immunohistochemistry direct western
TFEB deficiency attenuates mitochondrial degradation upon brown adipose tissue whitening at thermoneutrality.

PMID 33516944 · PMC7903014 · Molecular metabolism · 2021 · 7 claims · 8 setups

Has reproduction

BAT whitening at thermoneutrality is associated with TFEB-dependent mitochondrial degradation.

bat subscapular tfeb-bat-ko metabolically qrt-pcr western immunohistochemistry
Expression patterns of cardiac myofilament proteins: genomic and protein analysis of surgical myectomy tissue from patients with obstructive hypertrophic cardiomyopathy.

PMID 19808356 · PMC2765062 · Circulation. Heart failure · 2009 · 8 claims · 4 setups

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Myofilament protein levels (MYBPC3 and MYH7) are increased, not decreased, in HCM patients carrying a mutation in either gene compared to healthy heart tissue

genomic lysate formalin-fixed myectomy mutational western immunohistochemistry
Role of p16/MTS1, cyclin D1 and RB in primary oral cancer and oral cancer cell lines.

PMID 10389982 · PMC2363027 · British journal of cancer · 1999 · 7 claims · 5 setups

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p16/MTS1 mutations and loss of expression are very common in OSCC cell lines and less frequent in primary OSCC tumours

oscc same frozen pcr western extraction
Mitotic checkpoint protein hsMAD2 as a marker predicting liver metastasis of human gastric cancers.

PMID 11572763 · PMC5926839 · Japanese journal of cancer research : Gann · 2001 · 8 claims · 4 setups

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No mutations were found in the coding sequence of the hsMAD2 gene in 32 primary gastric cancers

gastric cancer formalin-fixed near-diploid pcr semi-quantitative immunohistochemistry
A candidate metastasis-associated DNA marker for ductal mammary carcinoma.

PMID 12631399 · PMC154149 · Breast cancer research : BCR · 2003 · 8 claims · 8 setups

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RDA comparing normal and metastatic ductal breast carcinoma cell DNA identified 10 unique metastasis-associated DNA sequences (MADS) apparently lost in metastatic cells

microdissected h&e-stained metastatic cloned representational single laser
Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-beta signaling.

PMID 19836472 · PMC2829334 · Biochimica et biophysica acta · 2010 · 8 claims · 7 setups

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Label-free 2D-LC-MS/MS quantitative proteomics identified 476 significantly differentially expressed proteins in kidney tubules of diabetic OVE26 mice versus control mice

kidney hk11 list 2d-lc-ms immunoblot immunohistochemistry
A protein-based set of reference markers for liver tissues and hepatocellular carcinoma.

PMID 19725976 · PMC2742551 · BMC cancer · 2009 · 7 claims · 5 setups

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Three protein spots (beta-actin, HSP60, PDI) show low expression variability across tumourous, non-tumourous cirrhotic, and normal liver tissues by 2-DE (ANOVA p>0.99 ranking)

liver 2-de western immunohistochemistry
The butyrophilin 1a1 knockout mouse revisited: Ablation of Btn1a1 leads to concurrent cell death and renewal in the mammary epithelium during lactation.

PMID 34938960 · PMC8664049 · FASEB bioAdvances · 2021 · 8 claims · 8 setups

Has reproduction · 98

BTN1A1 functions as an apical membrane receptor that forms a lipid-droplet secretion complex with the redox enzyme xanthine oxidoreductase (XDH)

mammary rna-seq proteomic qrt-pcr
Integrative analysis reveals chemokines CCL2 and CXCL5 mediated shear stress-induced aortic dissection formation.

PMID 38163105 · PMC10757018 · Heliyon · 2024 · 7 claims · 8 setups

Has reproduction · 75

Chemokines CCL2 and CXCL5 mediate a shear stress-induced 'Endothelial-Monocyte-Neutrophil' axis that contributes to aortic dissection development.

ascending aortic clinical bulk bulk single-cell western
IL-6 trans-Signaling Regulates Neutrophilic Inflammation in Alcohol-Associated Hepatitis.

PMID 40562277 · PMC13168973 · The American journal of pathology · 2026 · 8 claims · 8 setups

Has reproduction · 71

Hepatic IL-6R expression progressively declines with increasing severity of alcohol-related liver disease, from normal to early ASH to nonsevere and severe AH

liver hepatocytes hepg2 ptpn11 bulk immunohistochemistry single-cell
Microglial Fkbp5 Impairs Post-Stroke Vascular Integrity and Regeneration by Promoting Yap1-Mediated Glycolysis and Oxidative Phosphorylation.

PMID 41355597 · PMC13042415 · Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026 · 8 claims · 8 setups

Has reproduction · 50

A post-stroke perivascular microglial niche (stroke-VAM) exists, characterized by low M2 marker expression and elevated glycolysis, OXPHOS, and phagocytic activity.

ipsilateral cx3cr1cre peri-infarct ischemic single-cell single-nucleus immunofluorescence
Abnormalities of the p53 MDM2 and DCC genes in human leiomyosarcomas.

PMID 8198970 · PMC1969417 · British journal of cancer · 1994 · 6 claims · 8 setups

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A significant minority of leiomyosarcomas harbor p53 gene point mutations or deletions

leiomyosarcomas frozen leiomyosarcoma sarcoma sscp direct immunohistochemistry
The role of p53 inactivation in human cervical cell carcinoma development.

PMID 7841033 · PMC2033612 · British journal of cancer · 1995 · 8 claims · 7 setups

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HPV DNA sequences were detected in 43 of 47 (91.5%) primary uterine cervical cancers

formalin-fixed cervical p53 recurrent pcr southern pcr-single-strand
A proteomic approach to differentiate histologically classified stable and unstable plaques from human carotid arteries.

PMID 18715566 · PMC2659534 · Atherosclerosis · 2009 · 6 claims · 4 setups

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Stable and unstable carotid plaque extracts show distinct 2D-PAGE protein expression profiles, with nine proteins significantly differing in abundance between the two groups

carotid excised 2-dimensional maldi-tof western
Mutations in the C-terminus of the X protein of hepatitis B virus regulate Wnt-5a expression in hepatoma Huh7 cells: cDNA microarray and proteomic analyses.

PMID 18477650 · PMC2443277 · Carcinogenesis · 2008 · 8 claims · 7 setups

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HBx C-terminal deletion mutants (HBx3'-30 and HBx3'-40) produce differentially expressed gene and protein profiles in transfected Huh7 HCC cells, mostly involving transcriptional regulation, oncogenes/tumor suppressors, cell junctions, signal transduction, metabolism and immune response

huh7 frozen hcc genomic cdna two-dimensional western

Experiments

The use of laser capture microscopy in proteomics research--a review.

A missense mutation (Q279R) in the fumarylacetoacetate hydrolase gene, responsible for hereditary tyrosinemia, acts as a splicing mutation.

Molecular dissection of ALS-associated toxicity of SOD1 in transgenic mice using an exon-fusion approach.

Breast cancer proteomics reveals correlation between estrogen receptor status and differential phosphorylation of PGRMC1.

Expression of p73, a novel protein related to the p53 tumour suppressor p53, and apoptosis in cholangiocellular carcinoma of the liver.

TFEB deficiency attenuates mitochondrial degradation upon brown adipose tissue whitening at thermoneutrality.

Expression patterns of cardiac myofilament proteins: genomic and protein analysis of surgical myectomy tissue from patients with obstructive hypertrophic cardiomyopathy.

Role of p16/MTS1, cyclin D1 and RB in primary oral cancer and oral cancer cell lines.

Mitotic checkpoint protein hsMAD2 as a marker predicting liver metastasis of human gastric cancers.

A candidate metastasis-associated DNA marker for ductal mammary carcinoma.

Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-beta signaling.

A protein-based set of reference markers for liver tissues and hepatocellular carcinoma.

The butyrophilin 1a1 knockout mouse revisited: Ablation of Btn1a1 leads to concurrent cell death and renewal in the mammary epithelium during lactation.

Integrative analysis reveals chemokines CCL2 and CXCL5 mediated shear stress-induced aortic dissection formation.

IL-6 trans-Signaling Regulates Neutrophilic Inflammation in Alcohol-Associated Hepatitis.

Microglial Fkbp5 Impairs Post-Stroke Vascular Integrity and Regeneration by Promoting Yap1-Mediated Glycolysis and Oxidative Phosphorylation.

Abnormalities of the p53 MDM2 and DCC genes in human leiomyosarcomas.

The role of p53 inactivation in human cervical cell carcinoma development.

A proteomic approach to differentiate histologically classified stable and unstable plaques from human carotid arteries.

Mutations in the C-terminus of the X protein of hepatitis B virus regulate Wnt-5a expression in hepatoma Huh7 cells: cDNA microarray and proteomic analyses.