Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

Publications 1.3k Datasets 392.5k Experiments 30

System / cell line: gfp ✕ Assay: confocal ✕ Clear

Novel causative mutations in patients with Nance-Horan syndrome and altered localization of the mutant NHS-A protein isoform.

PMID 18949062 · PMC2571945 · Molecular vision · 2008 · 8 claims · 5 setups

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Truncating (nonsense/frameshift) mutations in NHS cause Nance-Horan syndrome by prematurely terminating the protein

genomic nhs-a hek mdck pcr pcr-based western
Mutations in the focal adhesion targeting region of deleted in liver cancer-1 attenuate their expression and function.

PMID 18829524 · PMC2597479 · Cancer research · 2008 · 8 claims · 6 setups

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The DLC-1 fragment spanning residues 201-500 is sufficient for focal adhesion targeting (FAT region)

a549 prostate colon mda-mb-468 immunofluorescence pcr colony
A new paradigm for MAPK: structural interactions of hERK1 with mitochondria in HeLa cells.

PMID 19847302 · PMC2760858 · PloS one · 2009 · 8 claims · 8 setups

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hERK1 translocates to the mitochondria of HeLa cells upon a proliferative stimulus

hela confocal immunofluorescence western
Localization studies of rare missense mutations in cystic fibrosis transmembrane conductance regulator (CFTR) facilitate interpretation of genotype-phenotype relationships.

PMID 18951463 · PMC2785447 · Human mutation · 2008 · 5 claims · 5 setups

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R1070P and R1070W CFTR mutants show apical membrane localization/insertion defects consistent with their associated disease severity

polarized mdck mdck-cftr patients confocal surface western
Point mutations in GLI3 lead to misregulation of its subcellular localization.

PMID 19829694 · PMC2758996 · PloS one · 2009 · 6 claims · 8 setups

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The MID1-α4-PP2A complex regulates the subcellular localization and transcriptional activity of GLI3.

hela immunofluorescence real-time luciferase
Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival.

PMID 36329028 · PMC9633600 · Nature communications · 2022 · 8 claims · 6 setups

Has reproduction · 87

A spatially resolved phosphoproteomics (SRP) approach combining APEX2-driven proximity biotinylation with phosphopeptide enrichment was developed to identify FGFR2b signalling partners near recycling endosomes.

hela_fgfr2bst hela-fgfr2b hela_fgfr2b-apex2st hela-fgfr2bst_rab11-apex2 confocal western ms-based
Characterization, expression profiles, intracellular distribution and association analysis of porcine PNAS-4 gene with production traits.

PMID 18588709 · PMC2464599 · BMC genetics · 2008 · 8 claims · 7 setups

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Porcine PNAS-4 encodes a 194-amino-acid protein that localizes to the Golgi complex

chinese tissues skeletal pk15 full-length real-time gfp
In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells.

PMID 32665556 · PMC7360550 · Nature communications · 2020 · 8 claims · 7 setups

Has reproduction · 50

Cancer cells in vivo display higher coherent orientation and alignment of MT dynamics along their cell major axis than 2D in vitro cultures, and distinct from 3D collagen gel cultures.

ht1080 es2 ht1080-eb3-mapple tumor intravital time-lapse confocal
Spns1-dependent endocardial lysosomal function drives valve morphogenesis through Notch1-signaling.

PMID 39720516 · PMC11667069 · iScience · 2024 · 7 claims · 8 setups

Has reproduction · 78

Autophagosomal, autolysosomal, and lysosomal vesicles significantly accumulate in the atrioventricular canal (AVC) and outflow tract (OFT)/bulboventricular regions and their developing valves during zebrafish heart development.

β-actin2 tgki cmv tgbac light-sheet lysotracker lysosome
Genetically engineered stem cell-derived retinal grafts for improved retinal reconstruction after transplantation.

PMID 34409267 · PMC8361135 · iScience · 2021 · 8 claims · 7 setups

Has reproduction · 84

Bhlhb4−/− and Islet1−/− grafts have markedly reduced bipolar cell populations after transplantation while retaining intact photoreceptor cell layers

bhlhb4− nrl-gfp rd1 transplanted retinal rt-pcr microarray
Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter.

PMID 18384686 · PMC2359743 · BMC cell biology · 2008 · 8 claims · 8 setups

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β1Int-HaloTag fusion protein localizes to the cell membrane in a pattern similar to endogenous β1 integrin, indicating the fusion does not disrupt normal integrin surface expression

hela hek293 live immunocytochemistry live fluorescence
Functional analysis of the novel TBX5 c.1333delC mutation resulting in an extended TBX5 protein.

PMID 18828908 · PMC2567295 · BMC medical genetics · 2008 · 8 claims · 6 setups

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c.1333delC is a novel de novo frameshift mutation in TBX5 exon 9 predicted to produce an elongated 580-amino-acid protein (74 miscoding + 62 supernumerary C-terminal residues) instead of a truncated one

peripheral cos-7 direct confocal co-localization
Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation.

PMID 37652021 · PMC10588993 · Immunity · 2023 · 7 claims · 8 setups

Has reproduction · 83

Mural cell-derived chemokines CCL2 and MIF preserve vascular macrophage survival and homeostatic function, generating a protective niche.

cx3cr1-mφca-rep mcrfp-rep cx3cr1-mφgfp-rep peritoneal multi-photon confocal imaging
Molecular dynamics and mutational analysis of a channelopathy mutation in the IIS6 helix of Ca V 1.2.

PMID 18836301 · PMC3196984 · Channels (Austin, Tex.) · 2008 · 8 claims · 4 setups

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I781T in CaV1.2 shifts the voltage-dependence of activation and inactivation to hyperpolarized voltages, reproducing the channelopathy-like gating phenotype of CaV1.4 I745T

homology tsa-201 cav1.2 molecular whole-cell confocal
Tbx5 drives Aldh1a2 expression to regulate a RA-Hedgehog-Wnt gene regulatory network coordinating cardiopulmonary development.

PMID 34643182 · PMC8555986 · eLife · 2021 · 8 claims · 8 setups

Has reproduction · 84

Tbx5 directly maintains Aldh1a2 expression in the foregut lateral plate mesoderm/pSHF via an evolutionarily conserved intronic enhancer.

micro-dissected dissected e9.5 xenopus bulk rt-qpcr whole-mount
A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation.

PMID 36149401 · PMC9576273 · eLife · 2022 · 8 claims · 8 setups

Has reproduction · 50

A C-terminal patch of the LRRK2 Armadillo domain (residues ~350–550, 'site #1') binds non-phosphorylated Rab29, Rab8A, and Rab10 with low-micromolar affinity

recombinant lrrk2 hela hek293t microscale confocal immunoblotting
Genomic analysis of a heterogeneous Mendelian phenotype: multiple novel alleles for inherited hearing loss in the Palestinian population.

PMID 16460646 · PMC3525152 · Human genomics · 2006 · 8 claims · 8 setups

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GJB2 (connexin 26) mutations account for hearing loss in only 17 of 156 families (11%), a smaller fraction than reported in other populations.

palestinian genomic probands lymphoblasts targeted sanger pcr
Proteomic screen defines the hepatocyte nuclear factor 1alpha-binding partners and identifies HMGB1 as a new cofactor of HNF1alpha.

PMID 18160415 · PMC2275099 · Nucleic acids research · 2008 · 8 claims · 8 setups

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HMGB1 is a novel HNF1α-interacting protein identified via a co-IP-MS screening strategy

hepg2 recombinant co-immunoprecipitation immunofluorescence gst
Identification of novel regulatory factor X (RFX) target genes by comparative genomics in Drosophila species.

PMID 17875208 · PMC2375033 · Genome biology · 2007 · 8 claims · 4 setups

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A subset of C. elegans DAF-19 target genes have Drosophila homologs that are also regulated by dRFX, showing conservation of the RFX regulatory cascade between the two species.

drosophila genome melanogaster real-time comparative gfp
The N-terminus and alpha-5, alpha-6 helices of the pro-apoptotic protein Bax, modulate functional interactions with the anti-apoptotic protein Bcl-xL.

PMID 17519046 · PMC1890283 · BMC cell biology · 2007 · 8 claims · 8 setups

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Deletion of the first 29 N-terminal amino acids (Bax 30-192) causes constitutive mitochondrial accumulation and high cytotoxicity that is poorly inhibited by Bcl-xL or Bcl-2.

jurkat drosophila hek apoptotic uas-gal4 western

Experiments

Novel causative mutations in patients with Nance-Horan syndrome and altered localization of the mutant NHS-A protein isoform.

Mutations in the focal adhesion targeting region of deleted in liver cancer-1 attenuate their expression and function.

A new paradigm for MAPK: structural interactions of hERK1 with mitochondria in HeLa cells.

Localization studies of rare missense mutations in cystic fibrosis transmembrane conductance regulator (CFTR) facilitate interpretation of genotype-phenotype relationships.

Point mutations in GLI3 lead to misregulation of its subcellular localization.

Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival.

Characterization, expression profiles, intracellular distribution and association analysis of porcine PNAS-4 gene with production traits.

In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells.

Spns1-dependent endocardial lysosomal function drives valve morphogenesis through Notch1-signaling.

Genetically engineered stem cell-derived retinal grafts for improved retinal reconstruction after transplantation.

Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter.

Functional analysis of the novel TBX5 c.1333delC mutation resulting in an extended TBX5 protein.

Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation.

Molecular dynamics and mutational analysis of a channelopathy mutation in the IIS6 helix of Ca V 1.2.

Tbx5 drives Aldh1a2 expression to regulate a RA-Hedgehog-Wnt gene regulatory network coordinating cardiopulmonary development.

A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation.

Genomic analysis of a heterogeneous Mendelian phenotype: multiple novel alleles for inherited hearing loss in the Palestinian population.

Proteomic screen defines the hepatocyte nuclear factor 1alpha-binding partners and identifies HMGB1 as a new cofactor of HNF1alpha.

Identification of novel regulatory factor X (RFX) target genes by comparative genomics in Drosophila species.

The N-terminus and alpha-5, alpha-6 helices of the pro-apoptotic protein Bax, modulate functional interactions with the anti-apoptotic protein Bcl-xL.