Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

• Differential expression analysis in single-cell and spatial RNA-seq without model assumptions.

  PMID 41980775 · PMC13198004 · Cell reports methods · 2026 · 7 claims · 4 setups

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  Common DGE analysis methods (Wilcoxon test, unweighted t-test, pseudo-bulk aggregation, SCTransform-style parametrization) rely on unnecessary simplifications and assumptions that are inconsistent with experimental data and cause false findings

  e18 simulated scrna-seq sprna-seq scrna-seq simulation count

• Deconvolving cell-type-specific gene expression profiles from bulk RNA-seq samples.

  PMID 41886524 · PMC13038110 · PLoS computational biology · 2026 · 8 claims · 6 setups

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  BLUE, a U-Net-based deep learning model with dual branches (U-Net for GEPs, MLP for proportions), accurately predicts cell-type proportions and cell-type-specific gene expression profiles from bulk RNA-seq.

  pbmc pancreatic aml scrna-seq-derived real scrna-seq

• Unraveling the timeline of gene expression: A pseudotemporal trajectory analysis of single-cell RNA sequencing data.

  PMID 37994351 · PMC10663991 · F1000Research · 2023 · 8 claims · 6 setups

  Has reproduction · 67

  A comprehensive open-source R workflow combining trajectory inference (monocle3) and pseudo-bulk time course analysis (edgeR) can be applied to multi-sample scRNA-seq data of the mouse mammary gland.

  mammary aggregated single-cell quality doublet

• HIV-seq reveals gene expression differences between HIV-transcribing cells from viremic and suppressed people with HIV.

  PMID 41776157 · PMC12957429 · Nature communications · 2026 · 7 claims · 8 setups

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  HIV-seq (10x 5' scRNA-seq spiked with HIV-specific capture primers targeting R-U5-pre-gag, pol, tat-rev exon2, Psi, and RRE) increases detection of HIV RNA+ cells and HIV transcripts per cell compared to conventional 5' scRNA-seq

  cd4+ cd8+ pbmcs scrna-seq cite-seq differential

• Unveiling gene perturbation effects through gene regulatory networks inference from single-cell transcriptomic data.

  PMID 41984780 · PMC13082667 · PLoS computational biology · 2026 · 7 claims · 4 setups

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  IGNITE is an unsupervised framework that infers directed, weighted, and signed GRNs directly from unperturbed scRNA-seq data using the inverse problem for a kinetic Ising model.

  pluripotent scrna-seq gene perturbation

• FEM: mining biological meaning from cell level in single-cell RNA sequencing data.

  PMID 34909283 · PMC8641482 · PeerJ · 2021 · 8 claims · 5 setups

  Has reproduction · 75

  The FEM algorithm converts a single-cell gene expression matrix into a functional expression matrix using multi-module gene set enrichment analysis, utilizing information from all expressed genes rather than discarding filtered genes.

  pbmc pancreas liver scrna-seq single-cell fisher's dimensionality

• TE-SCALE: a comprehensive database for exploring transposable element expression across human cancers at single-cell resolution.

  PMID 41296555 · PMC12807651 · Nucleic acids research · 2026 · 8 claims · 8 setups

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  TE-SCALE is a comprehensive single-cell database integrating TE expression across 20 human cancer types and 12 tissue origins

  tumor quality-controlled scrna-seq metacells scrna-seq subfamily automated

• The transcription factor HHEX maintains glucocorticoid levels and protects adrenals from androgen-induced lipid depletion.

  PMID 41519914 · PMC12891536 · Nature communications · 2026 · 8 claims · 8 setups

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  HHEX is the most enriched transcription factor in glucocorticoid-producing zona fasciculata (zF) cells, conserved across mouse, rat, and human adrenal

  sf1-cre-high adrenocortical scrna-seq rnascope immunohistochemistry

• Single-cell transcriptomics of acetaminophen-induced responses in human 2D and 3D liver microtissues.

  PMID 41535591 · PMC13043593 · Archives of toxicology · 2026 · 8 claims · 7 setups

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  3D liver spheroids exhibit greater transcriptional diversity and elevated ribosomal gene expression compared to 2D monolayers

  monolayer spheroid pooled single single-cell reactome hypoxia

• Ectopic NMDAR expression in cancer unmasks germline-encoded autoimmunity.

  PMID 41882353 · PMC13216075 · Nature · 2026 · 8 claims · 8 setups

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  GluN1 and GluN2B NMDAR subunits are ectopically expressed in cancer cells of human TNBC tumours

  breast tnbc 4t1-nmdar orthotopic bulk spatial immunofluorescence

• Single-cell RNA-seq reveals trans-sialidase-like superfamily gene expression heterogeneity in Trypanosoma cruzi populations.

  PMID 41945382 · PMC13056360 · eLife · 2026 · 8 claims · 5 setups

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  Surface protein-coding genes, especially TcS superfamily members, are expressed with greater heterogeneity than single-copy genes.

  population scrna-seq cruzi trypomastigote single-cell clustering bulk

• Benchmarking component choices for unpaired single cell RNA and epigenomic integration.

  PMID 41987329 · PMC13192178 · Genome biology · 2026 · 7 claims · 8 setups

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  Gene activity scores (GAS) show limited correlation with actual gene expression but effectively preserve cellular neighborhood structure and support clustering.

  e18 pbmc brain kidney scrna-seq share-seq gas

• Creation of a Single Cell RNASeq Meta-Atlas to Define Human Liver Immune Homeostasis.

  PMID 34335581 · PMC8322955 · Frontiers in immunology · 2021 · 7 claims · 8 setups

  Has reproduction · 63

  Three independently generated human liver CD45+ scRNA-seq datasets (LACEe, Lnb, LCD45e) can be co-clustered and integrated despite differing sequencing platforms and processing protocols.

  liver pbmc integrated gene scrna-seq differential ingenuity

• Allele-specific immune gene quantification and expression analysis in single-cell RNA-seq data.

  PMID 41229397 · PMC12604667 · NAR genomics and bioinformatics · 2025 · 8 claims · 4 setups

  Has reproduction · 75

  scIGD is a Snakemake workflow that automates HLA allele-typing and allele-specific expression quantification from scRNA-seq data

  aida merkel-cell 20k myeloma scrna-seq

• Cell type-specific eQTL analysis of COVID-19 based on single-cell transcriptomic data.

  PMID 41064594 · PMC12501775 · NAR genomics and bioinformatics · 2025 · 8 claims · 8 setups

  Has reproduction · 51

  Single-cell eQTL analysis across eight immune cell types identified 2593 genes whose expression is significantly associated with common genetic polymorphisms, with most genes showing cell type-specific effects

  pbmcs eight pbmc monocytes scrna-seq genetic cis-eqtl

• Spatial Transcriptomics of Human Decidua Identifies Molecular Signatures in Recurrent Pregnancy Loss.

  PMID 41031483 · PMC13242933 · Genomics, proteomics & bioinformatics · 2026 · 7 claims · 8 setups

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  The human decidua contains two coherent spatial domains, the implantation zone (IZ) and glandular-secretory zone (GZ), corresponding to the decidua compacta and spongiosa

  first-trimester decidual spots dnk 10x h&e type

• A new chromosome-level genome assembly for western painted turtle Chrysemys picta bellii, a model for extreme physiological adaptations.

  PMID 41792601 · PMC13077969 · BMC genomics · 2026 · 6 claims · 8 setups

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  A new haplotype-resolved, chromosome-level reference genome assembly (SLU_Cpb5.0) was generated for C. picta bellii using combined PacBio HiFi, 10x Genomics Chromium, Hi-C, and Bionano optical mapping data from a single individual.

  genomic ultra-high tissues heart 10x pacbio hi-c

• Peripheral Immune and Metabolic Dysregulation in Migraine, Ménière's Disease, and Vestibular Migraine: A Single-Cell Atlas Study.

  PMID 41953397 · PMC13054517 · Human mutation · 2026 · 8 claims · 8 setups

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  T cell abundance is significantly increased in MI compared to HC, while monocyte abundance is reduced, identifying T cells as a key pathogenic cell type

  pbmcs pbmc-derived degs pbmc single-cell differential kegg

• Deep single-cell decoding of human pancreatic islets reveals T2D β-cell gene expression defects.

  PMID 41986506 · PMC13226668 · The EMBO journal · 2026 · 8 claims · 6 setups

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  Single-cell transcriptome profiling of 245,878 islet cells from 48 donors (ND/PD/T2D) identifies 14 distinct, robust islet cell types detected in every donor

  pancreatic aggregated endoc-βh3 subset droplet-based pseudobulk unsupervised

• Large-scale integration of single-cell transcriptomic data captures transitional progenitor states in mouse skeletal muscle regeneration.

  PMID 34773081 · PMC8589952 · Communications biology · 2021 · 8 claims · 7 setups

  Has reproduction · 92

  Large-scale integration of 111 sc/snRNAseq datasets captures rare, transitional myogenic progenitor states (commitment and fusion) that are poorly represented in individual datasets.

  skeletal merged myogenic muscle scrnaseq batch-correction differential