Experiments
Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).
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A colorimetric method for point mutation detection using high-fidelity DNA ligase.
PMID 16257979 · PMC1275593 · Nucleic acids research · 2005 · 8 claims · 8 setups
High-fidelity Tth DNA ligase combined with allele-specific ligation-based gold nanoparticle assembly enables colorimetric single-base discrimination without precise temperature control
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Brain-specific proteins decline in the cerebrospinal fluid of humans with Huntington disease.
PMID 18984577 · PMC2649809 · Molecular & cellular proteomics : MCP · 2009 · 8 claims · 6 setups
Brain-specific proteins are 1.8 times more likely to be observed in CSF than in plasma
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Has reproduction · 70
Zebrafish functional xenograft vasculature platform identifies PF-502 as a durable vasculature normalization drug.
PMID 37680473 · PMC10480778 · iScience · 2023 · 8 claims · 8 setups
The zebrafish functional xenograft vasculature platform (zFXVP) enables visualization and quantification of structurally and functionally realistic tumor vasculature formation.
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Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
PMID 18683930 · PMC2649877 · Journal of the American Chemical Society · 2008 · 7 claims · 5 setups
Y289L GalT-mediated transfer of UDP-GalNAz followed by Cu(I)-catalyzed azide-alkyne cycloaddition enables selective, highly sensitive fluorescent/biotin labeling of O-GlcNAc-modified proteins
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A key role for mitochondria in endothelial signaling by plasma cysteine/cystine redox potential.
PMID 19879942 · PMC3057402 · Free radical biology & medicine · 2010 · 8 claims · 8 setups
Mitochondria are a major source of ROS in the endothelial signaling response to a more oxidized extracellular EhCySS
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A qualitative assessment of direct-labeled cDNA products prior to microarray analysis.
PMID 15762992 · PMC1079821 · BMC genomics · 2005 · 5 claims · 5 setups
The Agilent 2100 Bioanalyzer can be used in a novel assay to assess the quality/quantity of direct-labeled Cy-dye cDNA prior to microarray hybridization
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A novel approach to tag and identify geranylgeranylated proteins.
PMID 19784953 · PMC2855049 · Electrophoresis · 2009 · 7 claims · 7 setups
Metabolic incorporation of azido-GG alcohol followed by Cu(I)-catalyzed click reaction with TAMRA-alkyne selectively labels geranylgeranylated proteins for fluorescence detection
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Comparing protein abundance and mRNA expression levels on a genomic scale.
PMID 12952525 · PMC193646 · Genome biology · 2003 · 8 claims · 8 setups
Correlations between mRNA expression and protein abundance are generally poor or limited across most studies reviewed, including in yeast and human cancers
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Plasma proteomics of pancreatic cancer patients by multi-dimensional liquid chromatography and two-dimensional difference gel electrophoresis (2D-DIGE): up-regulation of leucine-rich alpha-2-glycoprotein in pancreatic cancer.
PMID 17303479 · PMC7105233 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2007 · 5 claims · 5 setups
Pre-fractionation of plasma with immuno-affinity depletion and anion-exchange chromatography prior to 2D-DIGE substantially increases the number of detectable protein spots compared with unfractionated plasma.
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Association of GSK3B with Alzheimer disease and frontotemporal dementia.
PMID 18852354 · PMC2841136 · Archives of neurology · 2008 · 8 claims · 5 setups
The GSK3B intronic polymorphism IVS2-68G>A is associated with increased risk of AD and FTD in a case-control cohort
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Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter.
PMID 18384686 · PMC2359743 · BMC cell biology · 2008 · 8 claims · 8 setups
β1Int-HaloTag fusion protein localizes to the cell membrane in a pattern similar to endogenous β1 integrin, indicating the fusion does not disrupt normal integrin surface expression
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The i-motif in the bcl-2 P1 promoter forms an unexpectedly stable structure with a unique 8:5:7 loop folding pattern.
PMID 19908860 · PMC2787777 · Journal of the American Chemical Society · 2009 · 8 claims · 6 setups
The full-length bcl-2 C-rich promoter sequence (Py39WT) forms one major intramolecular i-motif structure with a transitional pH of 6.6
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Has reproduction · 93
Experimental identification and in silico prediction of bacterivory in green algae.
PMID 33649548 · PMC8245530 · The ISME journal · 2021 · 7 claims · 6 setups
Five prasinophyte strains (Pterosperma cristatum NIES626, Pyramimonas parkeae CCMP726, Pyramimonas parkeae NIES254, Nephroselmis pyriformis RCC618, Dolichomastix tenuilepis CCMP3274) ingest live fluorescently labeled bacteria, detected by microscopy and/or flow cytometry
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From linear genome sequence to three-dimensional organization of the cell nucleus.
PMID 12620101 · PMC153456 · Genome biology · 2003 · 8 claims · 8 setups
Chromosome conformation capture (3C) can quantify in vivo physical interaction frequencies between genomic loci by crosslinking, digestion, and intramolecular ligation followed by PCR
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The regulation of endocytosis by kinases: cell biology meets genomics.
PMID 16420682 · PMC1414100 · Genome biology · 2005 · 8 claims · 4 setups
A genome-wide kinase RNAi screen reveals extensive and complex regulation of endocytosis by the human kinome.
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Viroporin potential of the lentivirus lytic peptide (LLP) domains of the HIV-1 gp41 protein.
PMID 18028545 · PMC2211469 · Virology journal · 2007 · 8 claims · 4 setups
Synthetic peptides corresponding to LLP-1, LLP-2, and LLP-3 domains partition into POPC:POPG lipid membranes and adopt amphipathic α-helical secondary structure
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Homozygous P86S mutation of the human glucagon receptor is associated with hyperglucagonemia, alpha cell hyperplasia, and islet cell tumor.
PMID 19657311 · PMC2767399 · Pancreas · 2009 · 8 claims · 6 setups
A homozygous P86S mutation in GCGR is associated with hyperglucagonemia and α cell hyperplasia in the patient
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Mutation in mitochondrial complex I ND6 subunit is associated with defective response to hypoxia in human glioma cells.
PMID 15248896 · PMC481082 · Molecular cancer · 2004 · 8 claims · 8 setups
An unreported T14634C mutation in the mtDNA-encoded ND6 subunit of Complex I is present in the hypoxia-sensitive glioma cell line M010b but not in hypoxia-tolerant lines.
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Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments.
PMID 15860768 · PMC1087789 · Nucleic acids research · 2005 · 8 claims · 4 setups
Selector oligonucleotides can circularize many distinct genomic restriction fragments in one reaction, enabling parallel amplification with a single universal primer pair instead of multiple target-specific primer pairs
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Competitive enzymatic reaction to control allele-specific extensions.
PMID 15767273 · PMC1065263 · Nucleic acids research · 2005 · 6 claims · 7 setups
Protease-mediated allele-specific extension (PrASE) uses competition between polymerase activity and Proteinase K-mediated polymerase degradation to allow extension of perfectly matched primers while eliminating slower mismatched primer extension.