Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

• Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA.

  PMID 19740104 · PMC3107988 · The FEBS journal · 2009 · 8 claims · 4 setups

  Full-text index only

  Human topoisomerase I (topo70) does not dimerize either free in solution or when covalently bound to DNA, ruling out dimerization as the source of a second DNA binding site

  gst-topo70 topo70 32p-labeled 3h-labeled gst gel 32p-labeled

• A novel HRAS substitution (c.266C>G; p.S89C) resulting in decreased downstream signaling suggests a new dimension of RAS pathway dysregulation in human development.

  PMID 22821884 · PMC4166655 · American journal of medical genetics. Part A · 2012 · 8 claims · 6 setups

  Has reproduction · 88

  A novel heterozygous HRAS c.266C>G (p.S89C) germline mutation was identified in two siblings with severe fetal hydrops/pleural effusion (Patient 1) and polyhydramnios/Dandy-Walker malformation (Patient 2).

  cos-7 parental hras genomic ras immunoblotting egf

• Analysis of the glutathione S-transferase (GST) gene family.

  PMID 15607001 · PMC3500200 · Human genomics · 2004 · 8 claims · 4 setups

  Full-text index only

  The complete human GST gene family comprises 16 genes in six subfamilies: alpha (GSTA), mu (GSTM), omega (GSTO), pi (GSTP), theta (GSTT) and zeta (GSTZ).

  genome gst gst-related gstk1 gene sequence phylogenetic

• An attenuated phenotype of Costello syndrome in three unrelated individuals with a HRAS c.179G>A (p.Gly60Asp) mutation correlates with uncommon functional consequences.

  PMID 25914166 · PMC4830354 · American journal of medical genetics. Part A · 2015 · 7 claims · 8 setups

  Has reproduction

  HRAS c.179G>A (p.Gly60Asp) causes an attenuated Costello syndrome phenotype without severe failure-to-thrive, intellectual disability, or cancer predisposition

  cos-7 transiently patients parental ras co-immunoprecipitation immunoblotting

• RIT1 controls actin dynamics via complex formation with RAC1/CDC42 and PAK1.

  PMID 29734338 · PMC5937737 · PLoS genetics · 2018 · 8 claims · 8 setups

  Has reproduction

  PAK1 is a novel direct effector of RIT1

  hek293t cos7 his-tagged western gst co-immunoprecipitation

• A common polymorphism in the oxygen-dependent degradation (ODD) domain of hypoxia inducible factor-1alpha (HIF-1alpha) does not impair Pro-564 hydroxylation.

  PMID 14521712 · PMC212228 · Molecular cancer · 2003 · 6 claims · 5 setups

  Full-text index only

  Pro582Ser is a common HIF-1α ODD-domain polymorphism occurring at similar frequency in idiopathic erythrocytosis patients (0.109) and normal controls (0.073)

  genomic recombinant 35s-labeled sanger pcr-rflp gst

• Mutations in the focal adhesion targeting region of deleted in liver cancer-1 attenuate their expression and function.

  PMID 18829524 · PMC2597479 · Cancer research · 2008 · 8 claims · 6 setups

  Full-text index only

  The DLC-1 fragment spanning residues 201-500 is sufficient for focal adhesion targeting (FAT region)

  a549 prostate colon mda-mb-468 immunofluorescence pcr colony

• A new paradigm for MAPK: structural interactions of hERK1 with mitochondria in HeLa cells.

  PMID 19847302 · PMC2760858 · PloS one · 2009 · 8 claims · 8 setups

  Full-text index only

  hERK1 translocates to the mitochondria of HeLa cells upon a proliferative stimulus

  hela confocal immunofluorescence western