Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

Publications 1.3k Datasets 392.5k Experiments 44

System / cell line: subcellular ✕ Assay: immunofluorescence ✕ Clear

LOCATE: a mammalian protein subcellular localization database.

PMID 17986452 · PMC2238969 · Nucleic acids research · 2008 · 8 claims · 6 setups

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LOCATE is a curated, web-accessible database housing membrane organization and subcellular localization data for mouse and human proteins.

hela proteome indirect automated computational
Point mutations in GLI3 lead to misregulation of its subcellular localization.

PMID 19829694 · PMC2758996 · PloS one · 2009 · 6 claims · 8 setups

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The MID1-α4-PP2A complex regulates the subcellular localization and transcriptional activity of GLI3.

hela immunofluorescence real-time luciferase
Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics.

PMID 12213833 · PMC2173151 · The Journal of cell biology · 2002 · 8 claims · 8 setups

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Subcellular proteomics of purified CCVs identifies enthoprotin (encoded by KIAA0171), a novel ENTH domain-containing protein not previously detected at the protein level.

brain cos-7 hek-293 clathrin subcellular western ccv
Disease-causing mutations in the CLRN1 gene alter normal CLRN1 protein trafficking to the plasma membrane.

PMID 19753315 · PMC2742642 · Molecular vision · 2009 · 8 claims · 5 setups

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Wild-type HA-tagged CLRN1 is correctly trafficked to the plasma membrane in transfected BHK-21 cells

bhk-21 genomic ush confocal western cycloheximide
Intracellular sorting and targeting of melanosomal membrane proteins: identification of signals for sorting of the human brown locus protein, gp75.

PMID 7642699 · PMC2199968 · The Journal of cell biology · 1995 · 7 claims · 7 setups

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The 36-amino acid cytoplasmic tail of gp75 alone is sufficient to direct intracellular retention and targeting of chimeric CD8 proteins to the endosomal/lysosomal compartment in fibroblasts

melanoma sk-mel-19 immunoprecipitated coli immunofluorescence immunoelectron protease
Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival.

PMID 36329028 · PMC9633600 · Nature communications · 2022 · 8 claims · 6 setups

Has reproduction · 87

A spatially resolved phosphoproteomics (SRP) approach combining APEX2-driven proximity biotinylation with phosphopeptide enrichment was developed to identify FGFR2b signalling partners near recycling endosomes.

hela_fgfr2bst hela-fgfr2b hela_fgfr2b-apex2st hela-fgfr2bst_rab11-apex2 confocal western ms-based
A quantitative proteomics analysis of subcellular proteome localization and changes induced by DNA damage.

PMID 20026476 · PMC2849709 · Molecular & cellular proteomics : MCP · 2010 · 6 claims · 5 setups

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A SILAC-based 'spatial proteomics' method can quantitatively measure the relative subcellular distribution of thousands of proteins across cytoplasm, nucleus, and nucleolus.

hct116 silac silac-based immunofluorescence
Multi-omic identification of perineurial hyperplasia and lipid-associated nerve macrophages in human polyneuropathies.

PMID 40849297 · PMC12375038 · Nature communications · 2025 · 8 claims · 8 setups

Has reproduction · 90

Multi-omic profiling (snRNA-seq + spatial transcriptomics) of human sural nerves identifies novel cell type markers (MLIP in mySC; GRIK3, PRIMA1 in nmSC; CXCL14 in periC) not previously described in rodent or human literature

sural peripheral snrna-seq nerve single-nucleus subcellular situ
Single-cell transcriptomics reveals maturation of transplanted stem cell-derived retinal pigment epithelial cells toward native state.

PMID 37339216 · PMC10293804 · Proceedings of the National Academy of Sciences of the United States of America · 2023 · 8 claims · 8 setups

Has reproduction · 59

Subretinally transplanted stem cell-derived RPE (ESC- and iPSC-derived) retain RPE identity and survive in vivo for at least 30 days in immunocompetent rabbit eyes

hpsc-derived day esc dutch qrt-pcr immunofluorescence transepithelial
The cell cycle and virus infection.

PMID 15576934 · PMC7120536 · Methods in molecular biology (Clifton, N.J.) · 2005 · 8 claims · 7 setups

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Viruses interfere with the host cell cycle to increase efficiency of virus replication

vero chick nucleoli mammalian flow indirect northern
Functional analysis of human mutations in homeodomain transcription factor PITX3.

PMID 17888164 · PMC2093940 · BMC molecular biology · 2007 · 7 claims · 5 setups

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Both S13N and G219fs mutants show partial loss-of-function in DNA-binding and/or transactivation activity, with G219fs more severely affected than S13N

lens western immunocytochemistry electrophoretic
Functional analysis of the novel TBX5 c.1333delC mutation resulting in an extended TBX5 protein.

PMID 18828908 · PMC2567295 · BMC medical genetics · 2008 · 8 claims · 6 setups

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c.1333delC is a novel de novo frameshift mutation in TBX5 exon 9 predicted to produce an elongated 580-amino-acid protein (74 miscoding + 62 supernumerary C-terminal residues) instead of a truncated one

peripheral cos-7 direct confocal co-localization
Identification of a dominant epitope of glutamic acid decarboxylase (GAD-65) recognized by autoantibodies in stiff-man syndrome.

PMID 8245784 · PMC2191306 · The Journal of experimental medicine · 1993 · 8 claims · 6 setups

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All 30 SMS patient sera selectively recognize GAD-65, but not GAD-67, when tested by Western blotting on denatured GAD.

brain bacterial triton cho western immunoprecipitation immunocytochemistry
A combined approach exploring gene function based on worm-human orthology.

PMID 15877817 · PMC1112593 · BMC genomics · 2005 · 8 claims · 6 setups

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Strict phylogenetic criteria (concordant Neighbor Joining and Maximum Parsimony tree topology) can select single most-likely human orthologs for C. elegans genes despite the large phylogenetic distance between worm and human sequences.

sapiens elegans hela phylogenetic rnai gfp
Quantitative membrane proteomics reveals new cellular targets of viral immune modulators.

PMID 17238276 · PMC1626102 · PLoS pathogens · 2006 · 8 claims · 8 setups

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SILAC-based quantitative membrane proteomics can identify novel targets of viral immunomodulators in an unbiased manner

hela silac immunoblot flow
The N-terminus and alpha-5, alpha-6 helices of the pro-apoptotic protein Bax, modulate functional interactions with the anti-apoptotic protein Bcl-xL.

PMID 17519046 · PMC1890283 · BMC cell biology · 2007 · 8 claims · 8 setups

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Deletion of the first 29 N-terminal amino acids (Bax 30-192) causes constitutive mitochondrial accumulation and high cytotoxicity that is poorly inhibited by Bcl-xL or Bcl-2.

jurkat drosophila hek apoptotic uas-gal4 western
The identification and characterization of a novel protein, c19orf10, in the synovium.

PMID 17362502 · PMC1906808 · Arthritis research & therapy · 2007 · 8 claims · 8 setups

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c19orf10 is a novel protein produced in significant amounts by fibroblast-like synoviocytes (FLSs), identified via proteomic analysis

fibroblast-like coli balb chicken two-dimensional recombinant monoclonal
Novel causative mutations in patients with Nance-Horan syndrome and altered localization of the mutant NHS-A protein isoform.

PMID 18949062 · PMC2571945 · Molecular vision · 2008 · 8 claims · 5 setups

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Truncating (nonsense/frameshift) mutations in NHS cause Nance-Horan syndrome by prematurely terminating the protein

genomic nhs-a hek mdck pcr pcr-based western
Human Proteinpedia: a unified discovery resource for proteomics research.

PMID 18948298 · PMC2686511 · Nucleic acids research · 2009 · 8 claims · 8 setups

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Human Proteinpedia is a community portal using a distributed annotation system (DAS) to share both published and unpublished human proteomic data

proteins tissues plasma expressing tandem immunohistochemistry mass
Characterization of novel isoforms and evaluation of SNF2L/SMARCA1 as a candidate gene for X-linked mental retardation in 12 families linked to Xq25-26.

PMID 18302774 · PMC2266716 · BMC medical genetics · 2008 · 8 claims · 4 setups

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The SNF2L gene spans 77 kb and is encoded by 25 exons that undergo alternative splicing to generate multiple distinct transcripts.

lines genomic 293hek rt-pcr genomic indirect

Experiments

LOCATE: a mammalian protein subcellular localization database.

Point mutations in GLI3 lead to misregulation of its subcellular localization.

Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics.

Disease-causing mutations in the CLRN1 gene alter normal CLRN1 protein trafficking to the plasma membrane.

Intracellular sorting and targeting of melanosomal membrane proteins: identification of signals for sorting of the human brown locus protein, gp75.

Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival.

A quantitative proteomics analysis of subcellular proteome localization and changes induced by DNA damage.

Multi-omic identification of perineurial hyperplasia and lipid-associated nerve macrophages in human polyneuropathies.

Single-cell transcriptomics reveals maturation of transplanted stem cell-derived retinal pigment epithelial cells toward native state.

The cell cycle and virus infection.

Functional analysis of human mutations in homeodomain transcription factor PITX3.

Functional analysis of the novel TBX5 c.1333delC mutation resulting in an extended TBX5 protein.

Identification of a dominant epitope of glutamic acid decarboxylase (GAD-65) recognized by autoantibodies in stiff-man syndrome.

A combined approach exploring gene function based on worm-human orthology.

Quantitative membrane proteomics reveals new cellular targets of viral immune modulators.

The N-terminus and alpha-5, alpha-6 helices of the pro-apoptotic protein Bax, modulate functional interactions with the anti-apoptotic protein Bcl-xL.

The identification and characterization of a novel protein, c19orf10, in the synovium.

Novel causative mutations in patients with Nance-Horan syndrome and altered localization of the mutant NHS-A protein isoform.

Human Proteinpedia: a unified discovery resource for proteomics research.

Characterization of novel isoforms and evaluation of SNF2L/SMARCA1 as a candidate gene for X-linked mental retardation in 12 families linked to Xq25-26.