Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

Publications 1.3k Datasets 392.5k Experiments 59

System / cell line: sds-page ✕ Assay: lc-ms ✕ Clear

Proteomic studies of cholangiocarcinoma and hepatocellular carcinoma cell secretomes.

PMID 20069059 · PMC2801507 · Journal of biomedicine & biotechnology · 2010 · 7 claims · 5 setups

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The secretomes of cholangiocarcinoma (HuCCA-1) and four hepatocellular carcinoma cell lines show distinct, differing protein profiles.

hucca-1 same conditioned paired sds-page trypan 1-de
Global mapping of the topography and magnitude of proteolytic events in apoptosis.

PMID 18724940 · PMC2597167 · Cell · 2008 · 7 claims · 6 setups

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PROTOMAP, a method combining 1D SDS-PAGE fractionation with LC-MS/MS-derived sequence coverage and spectral counting visualized as 'peptographs', enables global mapping of proteolytic topography and magnitude

jurkat sds-page fragmentation caspase-3
Comparative cytochrome P450 proteomics in the livers of immunodeficient mice using 18O stable isotope labeling.

PMID 17296599 · PMC2315784 · Molecular & cellular proteomics : MCP · 2007 · 8 claims · 5 setups

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SDS-PAGE combined with post-digest 18O/16O labeling and LC-MS/MS enables relative quantification of multiple P450 proteins from liver microsomes, including highly homologous isoforms

liver recombinant tryptic sds-page western mascot
Proteomic and phospho-proteomic profile of human platelets in basal, resting state: insights into integrin signaling.

PMID 19859549 · PMC2762604 · PloS one · 2009 · 8 claims · 8 setups

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A comprehensive platelet proteome of 1507 unique proteins was identified from ten independent human platelet samples, the most comprehensive platelet proteome assembled to date

platelets platelet anti-coagulated lc-ms phospho-proteomic membrane
Proteomic analyses of monocyte-derived macrophages infected with human immunodeficiency virus type 1 primary isolates from Hispanic women with and without cognitive impairment.

PMID 19115125 · PMC2947716 · Journal of neurovirology · 2009 · 7 claims · 5 setups

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HIV-1 isolates from CI patients induce a distinct macrophage proteome signature compared to isolates from NC patients and SF162

monocyte-derived mdm mdms western p24 seldi-tof sds-page
Proteomic-based identification of maternal proteins in mature mouse oocytes.

PMID 19646285 · PMC2730056 · BMC genomics · 2009 · 8 claims · 6 setups

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625 different proteins were identified from 2700 zona pellucida-free mature mouse MII oocytes, the largest oocyte proteome catalog to date

mature mii identified tissues sds-page acid gene
Identification of novel proteins affected by rotenone in mitochondria of dopaminergic cells.

PMID 17705834 · PMC2000881 · BMC neuroscience · 2007 · 6 claims · 5 setups

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SILAC-based quantitative proteomics combined with SDS-PAGE and LC-MS/MS can identify and quantify mitochondrial protein abundance changes in dopaminergic MES cells exposed to rotenone vs. control

mes silac western confocal
HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis.

PMID 14737186 · PMC314466 · PLoS biology · 2004 · 8 claims · 8 setups

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HIV-1 Nef binds the DOCK2-ELMO1 complex (which also contains Rac) in T cells

jurkat hek resting ns1 two-step ni-nta anti-flag
Preclinical and post-treatment changes in the HCC-associated serum proteome.

PMID 17060939 · PMC2360589 · British journal of cancer · 2006 · 7 claims · 6 setups

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SELDI proteomic profiling of serum reveals consistent, statistically significant changes associated with the development of HCC in hepatitis C patients

serum pooled excised seldi-tof anion lc-ms
Characterization of the placental macrophage secretome: implications for antiviral activity.

PMID 19070362 · PMC2947718 · Placenta · 2009 · 7 claims · 5 setups

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PM and MDM secrete different soluble proteins, some of which may contribute to decreased HIV-1 replication in PM

placental seldi-tof sds-page western
Application of proteomics methods for pathogen discovery.

PMID 19751767 · PMC7119679 · Journal of virological methods · 2010 · 8 claims · 6 setups

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Proteomic techniques can detect and characterize unknown infectious agents in cell culture without prior knowledge of the pathogen

hlf sequences synthetic conventional 2d-dige gelc-ms
Characterization of the 3a protein of SARS-associated coronavirus in infected vero E6 cells and SARS patients.

PMID 15312778 · PMC7127270 · Journal of molecular biology · 2004 · 8 claims · 7 setups

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ORF3a of SARS-CoV encodes an actual 31 kDa, 274-residue protein detected in infected cells and virions

vero crude sera sars-cov 2d-lc-ms sds-page western
Optimizing sample handling for urinary proteomics.

PMID 18662026 · PMC5451898 · Journal of proteome research · 2008 · 7 claims · 4 setups

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The method of protein extraction (lyophilization, ethanol precipitation, ultrafiltration, reverse-phase trapping column) does not alter the urinary proteome at the protein or peptide level

urine bradford sds-page
Proteomic profiling of amniotic fluid in preterm labor using two-dimensional liquid separation and mass spectrometry.

PMID 19012186 · PMC3163445 · The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2008 · 8 claims · 6 setups

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Amniotic fluid protein composition can be analyzed by combining 2D liquid chromatography and mass spectrometry to identify differentially expressed proteins in preterm labor

pooled amniotic two-dimensional sds-page maldi-tof
Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors.

PMID 16901338 · PMC1779605 · Genome biology · 2006 · 8 claims · 4 setups

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491 proteins were identified in human tear fluid using in-gel digestion and LC-MS/MS/MS3 on LTQ-FT and LTQ-Orbitrap instruments

closed-eye tear analysis sds-page in-solution gene
Cellular proteins in influenza virus particles.

PMID 18535660 · PMC2390764 · PLoS pathogens · 2008 · 8 claims · 6 setups

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Purified influenza virions contain 36 host-encoded cellular proteins in addition to the 9 previously known viral proteins.

influenza deglycosylated sucrose lc-ms mudpit electron
Optimization of protein solubilization for the analysis of the CD14 human monocyte membrane proteome using LC-MS/MS.

PMID 19709643 · PMC3159575 · Journal of proteomics · 2009 · 7 claims · 5 setups

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Methanol-based solubilization, alone or combined with PPS, yields significantly higher membrane protein identification/enrichment than PPS alone in monocyte membrane proteomics

bacteriorhodopsin crude immuno-affinity lc-ms sds-page shotgun facs
Large-scale and high-confidence proteomic analysis of human seminal plasma.

PMID 16709260 · PMC1779515 · Genome biology · 2006 · 8 claims · 6 setups

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923 proteins were identified with high confidence in seminal plasma from a single individual, combining results from three ejaculate samples

seminal same list combined sds-page database decoy
Pulmonary biomarkers based on alterations in protein expression after exposure to arsenic.

PMID 17450228 · PMC1852690 · Environmental health perspectives · 2007 · 5 claims · 6 setups

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Chronic low-dose As exposure (10/50 ppb) consistently alters expression of specific proteins (RAGE, GST-omega-1, contraspin, ApoA-I, ApoA-IV, peroxiredoxin-6, enolase-1) in mouse lung-lining fluid.

c57bl balf induced first 2-d multidimensional western
Proteomics-based identification of novel factor inhibiting hypoxia-inducible factor (FIH) substrates indicates widespread asparaginyl hydroxylation of ankyrin repeat domain-containing proteins.

PMID 18936059 · PMC2649815 · Molecular & cellular proteomics : MCP · 2009 · 8 claims · 5 setups

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DMOG pretreatment acts as a pharmacological 'substrate trap' that stabilizes transient FIH-substrate interactions, enabling their identification by SILAC-based proteomics

hek293 u2os 293t 293t-expressed co-immunoprecipitation silac-based co-immunoprecipitation-immunoblotting

Experiments

Proteomic studies of cholangiocarcinoma and hepatocellular carcinoma cell secretomes.

Global mapping of the topography and magnitude of proteolytic events in apoptosis.

Comparative cytochrome P450 proteomics in the livers of immunodeficient mice using 18O stable isotope labeling.

Proteomic and phospho-proteomic profile of human platelets in basal, resting state: insights into integrin signaling.

Proteomic analyses of monocyte-derived macrophages infected with human immunodeficiency virus type 1 primary isolates from Hispanic women with and without cognitive impairment.

Proteomic-based identification of maternal proteins in mature mouse oocytes.

Identification of novel proteins affected by rotenone in mitochondria of dopaminergic cells.

HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis.

Preclinical and post-treatment changes in the HCC-associated serum proteome.

Characterization of the placental macrophage secretome: implications for antiviral activity.

Application of proteomics methods for pathogen discovery.

Characterization of the 3a protein of SARS-associated coronavirus in infected vero E6 cells and SARS patients.

Optimizing sample handling for urinary proteomics.

Proteomic profiling of amniotic fluid in preterm labor using two-dimensional liquid separation and mass spectrometry.

Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors.

Cellular proteins in influenza virus particles.

Optimization of protein solubilization for the analysis of the CD14 human monocyte membrane proteome using LC-MS/MS.

Large-scale and high-confidence proteomic analysis of human seminal plasma.

Pulmonary biomarkers based on alterations in protein expression after exposure to arsenic.

Proteomics-based identification of novel factor inhibiting hypoxia-inducible factor (FIH) substrates indicates widespread asparaginyl hydroxylation of ankyrin repeat domain-containing proteins.