Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

Publications 1.3k Datasets 392.5k Experiments 17

System / cell line: over ✕ Assay: sds-page ✕ Clear

Global mapping of the topography and magnitude of proteolytic events in apoptosis.

PMID 18724940 · PMC2597167 · Cell · 2008 · 7 claims · 6 setups

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PROTOMAP, a method combining 1D SDS-PAGE fractionation with LC-MS/MS-derived sequence coverage and spectral counting visualized as 'peptographs', enables global mapping of proteolytic topography and magnitude

jurkat sds-page fragmentation caspase-3
Proteomic-based identification of maternal proteins in mature mouse oocytes.

PMID 19646285 · PMC2730056 · BMC genomics · 2009 · 8 claims · 6 setups

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625 different proteins were identified from 2700 zona pellucida-free mature mouse MII oocytes, the largest oocyte proteome catalog to date

mature mii identified tissues sds-page acid gene
Strategies for folding of affinity tagged proteins using GroEL and osmolytes.

PMID 19082872 · PMC3693453 · Journal of structural and functional genomics · 2009 · 8 claims · 8 setups

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GroEL/osmolyte mixtures can be used to refold difficult-to-fold chimeric affinity-tagged proteins by exploiting intrinsic chaperonin binding.

coli sds-page glutathione pepck-gst sds-page maldi-tof native
Localized-statistical quantification of human serum proteome associated with type 2 diabetes.

PMID 18795103 · PMC2529402 · PloS one · 2008 · 8 claims · 5 setups

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Developed LSPAD (localized statistics of protein abundance distribution) to calculate statistical significance of protein-abundance bias between two serum cohorts using a local Fisher's exact test window

pooled computational identified additional label-free lspad pathway-associated
Optimization of protein solubilization for the analysis of the CD14 human monocyte membrane proteome using LC-MS/MS.

PMID 19709643 · PMC3159575 · Journal of proteomics · 2009 · 7 claims · 5 setups

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Methanol-based solubilization, alone or combined with PPS, yields significantly higher membrane protein identification/enrichment than PPS alone in monocyte membrane proteomics

bacteriorhodopsin crude immuno-affinity lc-ms sds-page shotgun facs
Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors.

PMID 16901338 · PMC1779605 · Genome biology · 2006 · 8 claims · 4 setups

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491 proteins were identified in human tear fluid using in-gel digestion and LC-MS/MS/MS3 on LTQ-FT and LTQ-Orbitrap instruments

closed-eye tear analysis sds-page in-solution gene
Proteolysis of the endothelial cell protein C receptor by neutrophil proteinase 3.

PMID 17459006 · PMC1890847 · Journal of thrombosis and haemostasis : JTH · 2007 · 8 claims · 8 setups

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Activated neutrophils decrease EPCR surface expression on endothelial cells over time

ea.hy926 peripheral neutrophils recombinant flow luminol-dependent sds-page
Developmental and genetic regulation of human surfactant protein B in vivo.

PMID 18776725 · PMC2765709 · Neonatology · 2009 · 8 claims · 7 setups

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Pro-SP-B peptides are more common in developmentally less mature humans (amniotic fluid, neonatal tracheal aspirates) than in adults

amniotic tracheal bronchoalveolar simultaneous immunoblotting reducing gene
Proteomic analyses of monocyte-derived macrophages infected with human immunodeficiency virus type 1 primary isolates from Hispanic women with and without cognitive impairment.

PMID 19115125 · PMC2947716 · Journal of neurovirology · 2009 · 7 claims · 5 setups

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HIV-1 isolates from CI patients induce a distinct macrophage proteome signature compared to isolates from NC patients and SF162

monocyte-derived mdm mdms western p24 seldi-tof sds-page
N6-methyladenosine (m6A) reader Pho92 is recruited co-transcriptionally and couples translation to mRNA decay to promote meiotic fitness in yeast.

PMID 36422864 · PMC9731578 · eLife · 2022 · 8 claims · 8 setups

Has reproduction · 55

Pho92 specifically binds m6A-modified RNA via its YTH domain, both in vitro and in vivo

cerevisiae diploid yeast recombinant affinity rt-qpcr chip-seq
Proteomic analyses associate cystatin B with restricted HIV-1 replication in placental macrophages.

PMID 18951626 · PMC3867668 · Placenta · 2008 · 6 claims · 6 setups

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Placental macrophages support significantly lower HIV-1 replication than monocyte-derived macrophages

placental mdm hiv-1 seldi-tof sds-page
Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter.

PMID 18384686 · PMC2359743 · BMC cell biology · 2008 · 8 claims · 8 setups

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β1Int-HaloTag fusion protein localizes to the cell membrane in a pattern similar to endogenous β1 integrin, indicating the fusion does not disrupt normal integrin surface expression

hela hek293 live immunocytochemistry live fluorescence
Identification of serum biomarkers for colon cancer by proteomic analysis.

PMID 16755300 · PMC2361335 · British journal of cancer · 2006 · 8 claims · 8 setups

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Complement C3a des-arg, α1-antitrypsin and transferrin were identified as serum proteins with diagnostic potential for CRC.

serum pooled seldi seldi-tof seldi artificial
When does a protein become an allergen? Searching for a dynamic definition based on most advanced technology tools.

PMID 18477011 · PMC2607534 · Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2008 · 7 claims · 8 setups

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IgE-binding is not an intrinsic property of a protein but the result of an interaction between two molecules (antigen and IgE), so a molecule can only be classified relative to demonstrated IgE binding.

subjects allergic serum allergenic double-blind skin serum
Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis.

PMID 18795947 · PMC2585779 · The FEBS journal · 2008 · 8 claims · 8 setups

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Two late acyltransferase genes, lpxX and lpxL, were identified in M. catarrhalis O35E by informatics analysis of the partial genome sequence

lipid proteinase catarrhalis suspensions maldi-tof fatty sds-page
Congenital bovine spinal dysmyelination is caused by a missense mutation in the SPAST gene.

PMID 19714378 · PMC2854348 · Neurogenetics · 2010 · 8 claims · 5 setups

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A missense mutation (R560Q) in the SPAST gene's ATPase domain causes bovine spinal dysmyelination

cattle bovine coli affinity-purified snp reverse cdna
Spontaneous mutations in hlyD and tuf genes result in resistance of Dickeya solani IPO 2222 to phage ϕD5 but cause decreased bacterial fitness and virulence in planta.

PMID 37160956 · PMC10169776 · Scientific reports · 2023 · 7 claims · 8 setups

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Spontaneous ΦD5-resistant D. solani mutants DsR34 and DsR207 show significantly reduced virulence and colonization ability in planta compared to wild-type

potato solani planta maceration genome

Experiments

Global mapping of the topography and magnitude of proteolytic events in apoptosis.

Proteomic-based identification of maternal proteins in mature mouse oocytes.

Strategies for folding of affinity tagged proteins using GroEL and osmolytes.

Localized-statistical quantification of human serum proteome associated with type 2 diabetes.

Optimization of protein solubilization for the analysis of the CD14 human monocyte membrane proteome using LC-MS/MS.

Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors.

Proteolysis of the endothelial cell protein C receptor by neutrophil proteinase 3.

Developmental and genetic regulation of human surfactant protein B in vivo.

Proteomic analyses of monocyte-derived macrophages infected with human immunodeficiency virus type 1 primary isolates from Hispanic women with and without cognitive impairment.

N6-methyladenosine (m6A) reader Pho92 is recruited co-transcriptionally and couples translation to mRNA decay to promote meiotic fitness in yeast.

Proteomic analyses associate cystatin B with restricted HIV-1 replication in placental macrophages.

Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter.

Identification of serum biomarkers for colon cancer by proteomic analysis.

When does a protein become an allergen? Searching for a dynamic definition based on most advanced technology tools.

Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis.

Congenital bovine spinal dysmyelination is caused by a missense mutation in the SPAST gene.

Spontaneous mutations in hlyD and tuf genes result in resistance of Dickeya solani IPO 2222 to phage ϕD5 but cause decreased bacterial fitness and virulence in planta.