Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

Publications 1.3k Datasets 392.5k Experiments 33

System / cell line: serum ✕ Assay: stimulation ✕ Clear

A high-temporal resolution technology for dynamic proteomic analysis based on 35S labeling.

PMID 18714357 · PMC2500177 · PloS one · 2008 · 6 claims · 6 setups

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35S pulse-labeling (phosphor-imaging) detects more protein spots with higher sensitivity than CBB staining on the same 2-DE gels

a549 liver 35s electrophoresis paired
RIT1 controls actin dynamics via complex formation with RAC1/CDC42 and PAK1.

PMID 29734338 · PMC5937737 · PLoS genetics · 2018 · 8 claims · 8 setups

Has reproduction

PAK1 is a novel direct effector of RIT1

hek293t cos7 his-tagged western gst co-immunoprecipitation
Independent component analysis reveals new and biologically significant structures in micro array data.

PMID 16762055 · PMC1557674 · BMC bioinformatics · 2006 · 7 claims · 8 setups

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ICA applied to three microarray datasets reveals many biologically significant components, including low-ranking ones not obvious by rank alone

acute serum-stimulated head aml microarray clust term
Differential 14-3-3 affinity capture reveals new downstream targets of phosphatidylinositol 3-kinase signaling.

PMID 19648646 · PMC2773716 · Molecular & cellular proteomics : MCP · 2009 · 7 claims · 6 setups

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Four known insulin-regulated proteins (PFK-2, PRAS40, AS160, MYO1C) show high d0/d4 ratios, i.e. increased 14-3-3 binding upon insulin stimulation, validating the screen

hek293 hela far-western quantitative tio2
Investigating hookworm genomes by comparative analysis of two Ancylostoma species.

PMID 15854223 · PMC1112591 · BMC genomics · 2005 · 8 claims · 8 setups

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Nearly 20,000 ESTs from 7 cDNA libraries define nearly 7,000 hookworm genes across A. caninum and A. ceylanicum

caninum ceylanicum est bioinformatic functional
Leveraging two-way probe-level block design for identifying differential gene expression with high-density oligonucleotide arrays.

PMID 15099405 · PMC411067 · BMC bioinformatics · 2004 · 7 claims · 2 setups

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Two-way ANOVA and Mack-Skillings tests on probe-level data with FDR control are substantially more powerful than t-test/Wilcoxon on probe-set level data for detecting differential expression

fibroblast background high-density
A restricted spectrum of NRAS mutations causes Noonan syndrome.

PMID 19966803 · PMC3118669 · Nature genetics · 2010 · 8 claims · 6 setups

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Germline NRAS mutations (T50I, G60E) cause a subset of Noonan syndrome cases via enhanced MAPK activation

cohort cos-7 recombinant nras mutation western ras
Genome-wide location analysis and expression studies reveal a role for p110 CUX1 in the activation of DNA replication genes.

PMID 18003658 · PMC2248751 · Nucleic acids research · 2008 · 8 claims · 8 setups

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p110 CUX1 is recruited to promoters of cell cycle-related target genes preferentially during S phase

hela hs578t nih3t3 genomic chap-chip conventional scanning
Familial glucocorticoid deficiency with a point mutation in the ACTH receptor: a case report.

PMID 19795005 · PMC2752790 · Journal of Korean medical science · 2009 · 7 claims · 6 setups

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The patient has a homozygous D103N point mutation in the MC2R gene, with both parents heterozygous carriers

peripheral infant genomic serum rapid pcr
Two novel mutations in the aquaporin 2 gene in a girl with congenital nephrogenic diabetes insipidus.

PMID 16361827 · PMC2779314 · Journal of Korean medical science · 2005 · 8 claims · 5 setups

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The patient carries a compound heterozygous missense mutation in AQP2: A70D (exon 1, paternal) and R187H (exon 3, maternal)

3-year-old serum genomic clinical hormone water
Polymorphism in the tumour necrosis factor receptor II gene is associated with circulating levels of soluble tumour necrosis factor receptors in rheumatoid arthritis.

PMID 16277675 · PMC1297570 · Arthritis research & therapy · 2005 · 8 claims · 6 setups

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The TNF-RII T676G polymorphism is associated with circulating levels of sTNF-RI and sTNF-RII in RA, with a trend TT > TG > GG

serum genomic peripheral synovial elisa pcr-rflp clinical
Elevated serum levels of interferon-regulated chemokines are biomarkers for active human systemic lupus erythematosus.

PMID 17177599 · PMC1702557 · PLoS medicine · 2006 · 8 claims · 4 setups

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30 of 160 measured serum analytes (cytokines, chemokines, growth factors, soluble receptors) are dysregulated in SLE serum

serum blood peripheral custom luminex blood
Resistance to thyroid hormone with missense mutation (V349M) in the thyroid hormone receptor beta gene.

PMID 18363280 · PMC2686955 · The Korean journal of internal medicine · 2008 · 8 claims · 7 setups

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The patient's resistance to thyroid hormone (RTH) was caused by a novel missense mutation (V349M, c.1045G>A) in exon 11 of the TRβ gene, the first such case reported in Korea.

serum pituitary-thyroid thyroid sellar thyroid trh magnetic
Integrated analysis of genetic and proteomic data identifies biomarkers associated with adverse events following smallpox vaccination.

PMID 18923431 · PMC2692715 · Genes and immunity · 2009 · 7 claims · 6 setups

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A two-stage strategy (Random Forest filtering followed by decision tree modeling) can integrate categorical genetic and continuous proteomic data to identify biomarkers of AE risk

ebv-transformed serum combined rf-filtered snp serum random
A novel HRAS substitution (c.266C>G; p.S89C) resulting in decreased downstream signaling suggests a new dimension of RAS pathway dysregulation in human development.

PMID 22821884 · PMC4166655 · American journal of medical genetics. Part A · 2012 · 8 claims · 6 setups

Has reproduction · 88

A novel heterozygous HRAS c.266C>G (p.S89C) germline mutation was identified in two siblings with severe fetal hydrops/pleural effusion (Patient 1) and polyhydramnios/Dandy-Walker malformation (Patient 2).

cos-7 parental hras genomic ras immunoblotting egf
A new paradigm for MAPK: structural interactions of hERK1 with mitochondria in HeLa cells.

PMID 19847302 · PMC2760858 · PloS one · 2009 · 8 claims · 8 setups

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hERK1 translocates to the mitochondria of HeLa cells upon a proliferative stimulus

hela confocal immunofluorescence western
Cellular responses to ErbB-2 overexpression in human mammary luminal epithelial cells: comparison of mRNA and protein expression.

PMID 14710226 · PMC2395336 · British journal of cancer · 2004 · 7 claims · 5 setups

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There is a surprisingly high correlation between transcription and translation for the subset of genes studied by parallel microarray and proteomics analysis

hb4a serum-starved cdna 2d-difference immunoblotting
The oxazolidinone derivative locostatin induces cytokine appeasement.

PMID 19917702 · PMC2799496 · Journal of immunology (Baltimore, Md. : 1950) · 2009 · 7 claims · 7 setups

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RKIP is differentially localized (not differentially expressed) on the proteomic map after in vivo T cell recall compared to resting state

ot-i splenocytes fractions serum intracellular proteomics sds-page
An attenuated phenotype of Costello syndrome in three unrelated individuals with a HRAS c.179G>A (p.Gly60Asp) mutation correlates with uncommon functional consequences.

PMID 25914166 · PMC4830354 · American journal of medical genetics. Part A · 2015 · 7 claims · 8 setups

Has reproduction

HRAS c.179G>A (p.Gly60Asp) causes an attenuated Costello syndrome phenotype without severe failure-to-thrive, intellectual disability, or cancer predisposition

cos-7 transiently patients parental ras co-immunoprecipitation immunoblotting
A homozygous R262Q mutation in the gonadotropin-releasing hormone receptor presenting as constitutional delay of growth and puberty with subsequent borderline oligospermia.

PMID 16968799 · PMC1865483 · The Journal of clinical endocrinology and metabolism · 2006 · 7 claims · 8 setups

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A homozygous R262Q GNRHR mutation was identified in two brothers from an Asian Indian family, one of 11 families studied with delayed puberty or discordant IHH/delayed puberty siblings.

blood serum younger brothers mutational hormone 24-hour

Experiments

A high-temporal resolution technology for dynamic proteomic analysis based on 35S labeling.

RIT1 controls actin dynamics via complex formation with RAC1/CDC42 and PAK1.

Independent component analysis reveals new and biologically significant structures in micro array data.

Differential 14-3-3 affinity capture reveals new downstream targets of phosphatidylinositol 3-kinase signaling.

Investigating hookworm genomes by comparative analysis of two Ancylostoma species.

Leveraging two-way probe-level block design for identifying differential gene expression with high-density oligonucleotide arrays.

A restricted spectrum of NRAS mutations causes Noonan syndrome.

Genome-wide location analysis and expression studies reveal a role for p110 CUX1 in the activation of DNA replication genes.

Familial glucocorticoid deficiency with a point mutation in the ACTH receptor: a case report.

Two novel mutations in the aquaporin 2 gene in a girl with congenital nephrogenic diabetes insipidus.

Polymorphism in the tumour necrosis factor receptor II gene is associated with circulating levels of soluble tumour necrosis factor receptors in rheumatoid arthritis.

Elevated serum levels of interferon-regulated chemokines are biomarkers for active human systemic lupus erythematosus.

Resistance to thyroid hormone with missense mutation (V349M) in the thyroid hormone receptor beta gene.

Integrated analysis of genetic and proteomic data identifies biomarkers associated with adverse events following smallpox vaccination.

A novel HRAS substitution (c.266C>G; p.S89C) resulting in decreased downstream signaling suggests a new dimension of RAS pathway dysregulation in human development.

A new paradigm for MAPK: structural interactions of hERK1 with mitochondria in HeLa cells.

Cellular responses to ErbB-2 overexpression in human mammary luminal epithelial cells: comparison of mRNA and protein expression.

The oxazolidinone derivative locostatin induces cytokine appeasement.

An attenuated phenotype of Costello syndrome in three unrelated individuals with a HRAS c.179G>A (p.Gly60Asp) mutation correlates with uncommon functional consequences.

A homozygous R262Q mutation in the gonadotropin-releasing hormone receptor presenting as constitutional delay of growth and puberty with subsequent borderline oligospermia.