Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

• Biotin tagging coupled with amino acid-coded mass tagging for efficient and precise screening of interaction proteome in mammalian cells.

  PMID 19834888 · PMC4302342 · Proteomics · 2009 · 7 claims · 7 setups

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  BioCAT (biotin tagging + AACT) enables highly sensitive and accurate single-step screening of mammalian protein-protein interactions without establishing a stable cell line

  293t standard nk92mi list western sds-page nanolc-ms

• Distributed biotin-streptavidin transcription roadblocks for mapping cotranscriptional RNA folding.

  PMID 28398514 · PMC5499547 · Nucleic acids research · 2017 · 6 claims · 6 setups

  Has reproduction · 77

  A sequence-independent biotin–streptavidin (SAv) roadblocking strategy using randomly biotinylated DNA templates can stall TECs across all template positions for cotranscriptional SHAPE-Seq, simplifying template preparation and reducing cost.

  coli randomly radiolabeled cotranscriptional exonuclease

• A surrogate-based approach for post-genomic partner identification.

  PMID 11602024 · PMC57814 · BMC biotechnology · 2001 · 8 claims · 5 setups

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  Peptide surrogates derived from random phage display libraries contain amino acid sequence information that identifies the natural biological partner of the panned target via database searching.

  biotinylated tnf-β analysis secondary phage sequence secondary

• Glycan arrays for functional glycomics.

  PMID 12537579 · PMC151192 · Genome biology · 2002 · 6 claims · 5 setups

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  Glycan arrays allow high-throughput characterization of lectin binding specificity in a single experiment, improving on material-intensive hapten inhibition assays

  synthetic biotinylated mono monosaccharides neoglycolipid carbohydrate streptavidin-biotin

• Antibody binding loop insertions as diversity elements.

  PMID 17023486 · PMC1635297 · Nucleic acids research · 2006 · 7 claims · 8 setups

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  A lysozyme-binding VHH CDR3 loop can be grafted into two surface-exposed loops of superfolder GFP, conferring lysozyme-binding activity while the protein remains fluorescent.

  gfp-anti-lysozyme coli full-length gfp-hcdr3 elisa flow surface

• SNP-specific extraction of haplotype-resolved targeted genomic regions.

  PMID 18611953 · PMC2528194 · Nucleic acids research · 2008 · 6 claims · 4 setups

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  SNP-specific extraction isolates haploid genomic DNA flanking targeted SNPs by hybridizing an allele-specific oligo, enzymatically extending it with biotinylated nucleotides, and capturing the tagged allele with streptavidin-coated magnetic particles.

  genomic diploid quantitative sanger low

• Three assays show differences in binding of wild-type and mutant p53 to unique gene sequences.

  PMID 19925028 · PMC2917581 · Technology in cancer research & treatment · 2009 · 7 claims · 6 setups

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  Three different DNA binding assays (EMSA, SPA, streptavidin magnetic bead assay) show differences in binding of wild-type and mutant p53 to unique gene sequences

  nuclear wro electrophoretic binding streptavidin

• Protein microarray technology.

  PMID 17126887 · PMC1828913 · Mechanisms of ageing and development · 2007 · 8 claims · 8 setups

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  Protein microarrays enable high-throughput characterization of protein biochemical activities across an entire proteome in a single experiment

  yeast sh2 protein-protein protein-dna chromatin

• PathogenMIPer: a tool for the design of molecular inversion probes to detect multiple pathogens.

  PMID 17105657 · PMC1657037 · BMC bioinformatics · 2006 · 6 claims · 5 setups

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  PathogenMIPer designs unique, target-specific MIP probes, assembling all probe components (target-specific sequences, barcodes, universal primers, restriction sites) into ready-to-order probes for any genome.

  hpv clinical biotinylated pcr mip molecular microarray

• Human spermatozoa contain multiple targets for protein S-nitrosylation: an alternative mechanism of the modulation of sperm function by nitric oxide?

  PMID 17683036 · PMC2777308 · Proteomics · 2007 · 7 claims · 5 setups

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  Human sperm proteins undergo S-nitrosylation upon exposure to NO donors, detectable by the biotin switch assay

  percoll-washed fixed pooled excised biotin situ streptavidin-agarose

• Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival.

  PMID 36329028 · PMC9633600 · Nature communications · 2022 · 8 claims · 6 setups

  Has reproduction · 87

  A spatially resolved phosphoproteomics (SRP) approach combining APEX2-driven proximity biotinylation with phosphopeptide enrichment was developed to identify FGFR2b signalling partners near recycling endosomes.

  hela_fgfr2bst hela-fgfr2b hela_fgfr2b-apex2st hela-fgfr2bst_rab11-apex2 confocal western ms-based

• Mutations of the Igbeta gene cause agammaglobulinemia in man.

  PMID 17709424 · PMC2118692 · The Journal of experimental medicine · 2007 · 6 claims · 5 setups

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  A homozygous nonsense mutation (Gln80X) in the Igβ (B29) gene causes agammaglobulinemia in a human patient

  genomic drosophila pbmcs sorted pcr transfection flow

• Methods for the proteomic identification of protease substrates.

  PMID 19729334 · PMC2787889 · Current opinion in chemical biology · 2009 · 8 claims · 8 setups

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  Gel-based methods (2D-DiGE, diagonal electrophoresis, PROTOMAP) identify protease substrates by comparing proteolyzed versus control samples via electrophoretic migration differences followed by MS identification

  lysate jurkat apoptotic yeast 2d-dige protomap cofradic