Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

• The N-terminus and alpha-5, alpha-6 helices of the pro-apoptotic protein Bax, modulate functional interactions with the anti-apoptotic protein Bcl-xL.

  PMID 17519046 · PMC1890283 · BMC cell biology · 2007 · 8 claims · 8 setups

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  Deletion of the first 29 N-terminal amino acids (Bax 30-192) causes constitutive mitochondrial accumulation and high cytotoxicity that is poorly inhibited by Bcl-xL or Bcl-2.

  jurkat drosophila hek apoptotic uas-gal4 western

• The impact of peptide abundance and dynamic range on stable-isotope-based quantitative proteomic analyses.

  PMID 18798661 · PMC2746028 · Journal of proteome research · 2008 · 8 claims · 7 setups

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  Over half of confidently identified peptides in complex mixtures have S/N ratios below 10 on both FT-ICR and Orbitrap instruments

  jurkat silac-based sds-page database

• Novel peptide identification from tandem mass spectra using ESTs and sequence database compression.

  PMID 17437027 · PMC1865584 · Molecular systems biology · 2007 · 7 claims · 6 setups

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  Traditional protein-sequence-database search engines fail to identify peptides from alternative splicing and coding SNP isoforms despite acquisition of good-quality tandem mass spectra

  jurkat peptideatlas erythroleukemia plasma lc-ms

• β-Catenin activity induces an RNA biosynthesis program promoting therapy resistance in T-cell acute lymphoblastic leukemia.

  PMID 36597789 · PMC9906382 · EMBO molecular medicine · 2023 · 8 claims · 8 setups

  Has reproduction · 65

  β-catenin binds directly to promoters of RNA processing, splicing, and ribosomal biogenesis genes in T-ALL cells

  rpmi8402 chipseq chip-qpcr sequencing

• HIT: a versatile proteomics platform for multianalyte phenotyping of cytokines, intracellular proteins and surface molecules.

  PMID 18849997 · PMC3334282 · Nature medicine · 2008 · 8 claims · 8 setups

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  HIT uses oligonucleotide-tagged (Fab- or mSA-conjugated) antibodies, T7 polymerase amplification, and DNA microarray hybridization to indirectly measure multiple analytes in a fluid-phase multiplex format

  hsp70-coated hsp70 secreted jurkat fluid-phase multiplex hit

• Phosphoproteomics by mass spectrometry: insights, implications, applications and limitations.

  PMID 19929607 · PMC2931417 · Expert review of proteomics · 2009 · 8 claims · 8 setups

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  Serine/threonine phosphorylation widely functions to modulate protein-protein interactions (PPIs) across signaling systems

  hela jurkat exposed yeast quantitative antibody-based analog-sensitive

• Control of gene expression during T cell activation: alternate regulation of mRNA transcription and mRNA stability.

  PMID 15907206 · PMC1156890 · BMC genomics · 2005 · 8 claims · 5 setups

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  Regulation of mRNA stability accounts for as much as 50% of all measured changes in polyA mRNA levels, inferred from absence of corresponding nuclear transcription changes.

  jurkat nuclear polya single

• A reliable method to display authentic DNase I hypersensitive sites at long-ranges in single-copy genes from large genomes.

  PMID 16510851 · PMC1388096 · Nucleic acids research · 2006 · 6 claims · 3 setups

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  MDHA extends the range of classical DHA from ~20 kb increments to intervals approaching 100 kb using agarose-embedded nuclei, FIGE, and long-range Southern blotting

  jurkat specified mdha northern

• De novo identification of CD4(+) T cell epitopes.

  PMID 38658646 · PMC11093748 · Nature methods · 2024 · 7 claims · 8 setups

  Has reproduction · 83

  SABR-IIs (chimeric receptors linking a covalently attached peptide-MHC-II to CD28-CD3ζ signaling domains) present epitopes to CD4+ T cells and induce a readable NFAT-GFP/CD69 signal upon cognate TCR recognition

  nfat-gfp hla-dq8 5kc daudi nfat-gfp co-incubation species

• Depletion of HIV reservoir by activation of ISR signaling in resting CD4(+)T cells.

  PMID 36590168 · PMC9800255 · iScience · 2023 · 8 claims · 8 setups

  Has reproduction · 89

  Activation of ISR/ATF4 signaling (via HA15) reverses HIV latency in Jurkat models of HIV latency (J-Lat A1, 2D10)

  jurkat cd4+ hiv-negative resting flow trypan western

• CTCF binding site classes exhibit distinct evolutionary, genomic, epigenomic and transcriptomic features.

  PMID 19922652 · PMC3091324 · Genome biology · 2009 · 8 claims · 8 setups

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  CTCF binding sites can be classified into three occupancy-based classes (LowOc, MedOc, HighOc) based on similarity to the CTCF PWM motif

  cd4+ hela imr90 embryonic chip-seq computational gene

• The interleukin 12 p40 gene promoter is primed by interferon gamma in monocytic cells.

  PMID 8551218 · PMC2192398 · The Journal of experimental medicine · 1996 · 8 claims · 8 setups

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  IFN-gamma primes phagocytic cells for LPS-induced transcription of the IL-12 p40 gene rather than directly inducing it

  pbmc cell-depleted raw264.7 nuclei ria immunoprecipitation rnase