Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

• Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets.

  PMID 36513983 · PMC9749362 · BMC bioinformatics · 2022 · 8 claims · 7 setups

  Has reproduction · 99

  Long-read classifiers generally performed best among the 11 methods tested

  atcc zymobiomics taxonomic short-read

• The selection of software and database for metagenomics sequence analysis impacts the outcome of microbial profiling and pathogen detection.

  PMID 37027361 · PMC10081788 · PloS one · 2023 · 7 claims · 7 setups

  Has reproduction · 86

  Obtaining an accurate species-level microbial profile using current direct-read metagenomics profiling software is still a challenging task.

  simulated kidney biological extracted shotgun taxonomic pathogen

• Large-scale estimation of bacterial and archaeal DNA prevalence in metagenomes reveals biome-specific patterns.

  PMID 41854267 · PMC13098197 · mSystems · 2026 · 8 claims · 6 setups

  Full-text index only

  SPF scalably and robustly estimates the fraction of bacterial and archaeal reads in a metagenome using detection of prokaryotic single-copy marker genes, without requiring eukaryotic or viral reference genomes

  zymo cami simulated fecal simulated shotgun spf

• MetaPepticon: automated prediction of anticancer peptides from microbial genomes and metagenomes.

  PMID 41918857 · PMC13034871 · PeerJ · 2026 · 7 claims · 6 setups

  Full-text index only

  MetaPepticon is a modular, end-to-end Snakemake pipeline that predicts ACP candidates directly from raw genomic, metagenomic, transcriptomic, metatranscriptomic reads, assembled contigs, or peptide sequences.

  validated gut representative predicted acp shotgun genome

• RiboTaxa: combined approaches for rRNA genes taxonomic resolution down to the species level from metagenomics data revealing novelties.

  PMID 36159175 · PMC9492272 · NAR genomics and bioinformatics · 2022 · 8 claims · 6 setups

  Has reproduction · 66

  RiboTaxa, combining BBTools, FastQC, SortMeRNA, MetaRib, EMIRGE, VSEARCH, BBMap and QIIME 2's Sklearn classifier, was built as a pipeline for SSU rRNA-based taxonomic profiling of metagenomics data.

  microbial reconstructed synthetic real shotgun ssu full-length

• Wochenende - modular and flexible alignment-based shotgun metagenome analysis.

  PMID 36368923 · PMC9650795 · BMC genomics · 2022 · 8 claims · 6 setups

  Has reproduction · 88

  Wochenende is a modular, transparent alignment-based pipeline for shotgun metagenome analysis supporting short and long reads across all kingdoms of life

  zymo bacterial reference shotgun comparative reference

• MACSIMS: multiple alignment of complete sequences information management system.

  PMID 16792820 · PMC1539025 · BMC bioinformatics · 2006 · 8 claims · 5 setups

  Full-text index only

  MACSIMS is a multiple alignment-based information management system combining knowledge-based database mining with ab initio sequence predictions

  balibase-derived sequences same query automatic database initio

• Metagenomic study of the oral microbiota by Illumina high-throughput sequencing.

  PMID 19796657 · PMC3568755 · Journal of microbiological methods · 2009 · 8 claims · 6 setups

  Full-text index only

  The 16S rRNA V5 hypervariable region, amplified as a short ~82-base segment, provides reliable taxonomic identification of oral bacteria against public databases like HOMD.

  pooled 16s sequenced topo 16s primer taxonomic

• nf-core/mag: a best-practice pipeline for metagenome hybrid assembly and binning.

  PMID 35118380 · PMC8808542 · NAR genomics and bioinformatics · 2022 · 8 claims · 7 setups

  Has reproduction · 100

  nf-core/mag is a Nextflow/nf-core pipeline for hybrid metagenome assembly, binning and taxonomic classification of MAGs.

  simulated assembled mags raw metagenomic novo genome

• A step forward for Shiga toxin-producing Escherichia coli identification and characterization in raw milk using long-read metagenomics.

  PMID 36748417 · PMC9836091 · Microbial genomics · 2022 · 8 claims · 6 setups

  Has reproduction · 75

  Long-read metagenomics enables isolation-independent identification and characterization of eae-positive STEC directly from raw milk.

  pure enriched stec-negative extracted ont real-time artificial

• Draft Genome Sequences of Antimicrobial-Resistant Shigella Clinical Isolates from Pakistan.

  PMID 31346012 · PMC6658682 · Microbiology resource announcements · 2019 · 6 claims · 7 setups

  Has reproduction · 92

  Draft genome sequences of three multidrug-resistant Shigella clinical isolates from Pakistan (two S. flexneri, one S. sonnei) are reported as a resource for AMR surveillance.

  shigella bacterial antimicrobial whole-genome

• Full-length 16S rRNA nanopore sequencing enables species resolution of Fusobacterium associated with colorectal cancer.

  PMID 41963777 · PMC13078227 · Gut microbes · 2026 · 8 claims · 7 setups

  Full-text index only

  Full-length 16S rRNA ONT sequencing combined with custom demultiplexing (nanoMux) enables robust species-level discrimination within the Fusobacterium genus

  gtdb rrndb zymobiomics host-bacteria ani phylogenetic 16s