Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

• Identification of novel citrullinated autoantigens of synovium in rheumatoid arthritis using a proteomic approach.

  PMID 17125526 · PMC1794520 · Arthritis research & therapy · 2006 · 8 claims · 6 setups

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  51 citrullinated protein spots were detected in RA synovial tissue, of which 30 (58.8%) were autoantigenic (reactive with RA sera)

  synovial excised coli recombinant two-dimensional maldi-tof recombinant

• Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.

  PMID 18683930 · PMC2649877 · Journal of the American Chemical Society · 2008 · 7 claims · 5 setups

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  Y289L GalT-mediated transfer of UDP-GalNAz followed by Cu(I)-catalyzed azide-alkyne cycloaddition enables selective, highly sensitive fluorescent/biotin labeling of O-GlcNAc-modified proteins

  α-crystallin forebrain hela chemoenzymatic in-gel nanolc-ms

• Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens.

  PMID 18552983 · PMC2426731 · Molecular vision · 2008 · 7 claims · 6 setups

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  Six novel candidate retinal autoantigens (β-actin, esterase D, tubulin β-2, brain-type creatine kinase, VDAC, aspartate aminotransferase) were identified by 2D-WB and mass spectrometry in EAU mice

  c57bl retinal recombinant eau two-dimensional maldi-tof one-dimensional

• Proteomic identification of nitrated brain proteins in amnestic mild cognitive impairment: a regional study.

  PMID 17760844 · PMC3823261 · Journal of cellular and molecular medicine · 2007 · 8 claims · 5 setups

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  Total protein nitration (3-NT immunoreactivity) is significantly increased in MCI hippocampus and IPL compared to age-matched control brain

  postmortem excised mci 2d-page sypro in-gel

• Human spermatozoa contain multiple targets for protein S-nitrosylation: an alternative mechanism of the modulation of sperm function by nitric oxide?

  PMID 17683036 · PMC2777308 · Proteomics · 2007 · 7 claims · 5 setups

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  Human sperm proteins undergo S-nitrosylation upon exposure to NO donors, detectable by the biotin switch assay

  percoll-washed fixed pooled excised biotin situ streptavidin-agarose

• Using proteomic approach to identify tumor-associated antigens as markers in hepatocellular carcinoma.

  PMID 18672925 · PMC2680441 · Journal of proteome research · 2008 · 8 claims · 6 setups

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  34 immunoreactive protein spots were detected in 2DE Western blots probed with HCC patient sera but not normal sera

  hepg2 excised identified recombinant two-dimensional western in-gel

• Reduced expression of Axin correlates with tumour progression of oesophageal squamous cell carcinoma.

  PMID 12771989 · PMC2377153 · British journal of cancer · 2003 · 7 claims · 6 setups

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  Reduced Axin expression is correlated with tumour progression of oesophageal SCC

  surgical oesophageal excised clinical immunohistochemistry western northern

• CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles.

  PMID 17111436 · PMC2978322 · Proteomics · 2006 · 7 claims · 4 setups

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  CyDye immunoblotting (ECL Plex) allows simultaneous co-detection of total protein profile and a specific immunoreactive protein on the same membrane

  left tryptic 1-d 2-de maldi-tof

• Proteomic identification of non-Gal antibody targets after pig-to-primate cardiac xenotransplantation.

  PMID 18957049 · PMC2586876 · Xenotransplantation · 2008 · 8 claims · 6 setups

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  Non-Gal antibody response after cardiac xenotransplantation is directed to a diverse set of stress response, inflammation-related, cytoskeletal and metabolic pig EC antigens, with several immunodominant targets remaining undefined

  gt-ko spots explanted flow western nanolc

• Proteomic analyses of the effects of drugs of abuse on monocyte-derived mature dendritic cells.

  PMID 19811410 · PMC2812871 · Immunological investigations · 2009 · 6 claims · 7 setups

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  Cocaine differentially regulates expression of several functional classes of proteins (apoptosis, protein folding, kinase activity, metabolism, signal transduction) in monocyte-derived mature dendritic cells

  monocyte-derived excised difference hplc-tandem western

• Modeling substance abuse for applications in proteomics.

  PMID 20058165 · PMC3272758 · Methods in molecular biology (Clifton, N.J.) · 2009 · 8 claims · 5 setups

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  The intravenous self-administration paradigm models the reinforcing effects of abused drugs and allows biochemical alterations to be correlated with drug reinforcement.

  jugular non-human monkey spots intravenous infrared fractionation

• Proteomic identification of secreted proteins as surrogate markers for signal transduction inhibitor activity.

  PMID 17211472 · PMC2360009 · British journal of cancer · 2007 · 5 claims · 5 setups

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  Inhibiting EGFR signalling with Gefitinib alters the pattern of proteins secreted by cancer cells, and these changes can serve as an indirect quantitative assay for drug action

  a431 a549 excised gel maldi-tof western

• A proteomic approach to differentiate histologically classified stable and unstable plaques from human carotid arteries.

  PMID 18715566 · PMC2659534 · Atherosclerosis · 2009 · 6 claims · 4 setups

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  Stable and unstable carotid plaque extracts show distinct 2D-PAGE protein expression profiles, with nine proteins significantly differing in abundance between the two groups

  carotid excised 2-dimensional maldi-tof western

• Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference.

  PMID 18445260 · PMC2386481 · BMC cancer · 2008 · 8 claims · 5 setups

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  Stable RNAi-mediated MBD1 knock-down was successfully established in the BxPC-3 pancreatic cancer cell line using a recombinant siRNA plasmid

  bxpc-3 2-d maldi-tof-ms western

• In vivo pharmaco-proteomic analysis of hydroxyurea induced changes in the sickle red blood cell membrane proteome.

  PMID 19914412 · PMC2818491 · Journal of proteomics · 2010 · 8 claims · 4 setups

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  2D-DIGE combined with tandem mass spectrometry identified 32 distinct sickle RBC membrane proteins that significantly changed in abundance after in vivo HU therapy

  sickle excised patients 2d-dige tandem immunoblot

• Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma.

  PMID 19848416 · PMC2789179 · Journal of proteome research · 2009 · 6 claims · 6 setups

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  Transgelin (TAGLN), transgelin-2 (TAGLN2), and cyclophilin A (PPIA) are overexpressed in lung adenocarcinoma tissue compared to matched normal lung

  matched excised additional formalin-fixed 2-d lc-ms quantitative

• Synaptic proteins linked to HIV-1 infection and immunoproteasome induction: proteomic analysis of human synaptosomes.

  PMID 19693676 · PMC2824116 · Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2010 · 8 claims · 7 setups

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  Proteomic screening of human synaptosomes identifies a set of proteins differentially expressed in HIV/AIDS brain

  frontal excised synaptosome paraffin-embedded 2d-page maldi-tof western

• Proteomic-based identification of haptoglobin-1 precursor as a novel circulating biomarker of ovarian cancer.

  PMID 15199385 · PMC2364749 · British journal of cancer · 2004 · 7 claims · 7 setups

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  Six serum protein spots (~40 kDa, pI 5.9–6.6) significantly overexpressed in grade 1, 2 and 3 ovarian cancer patients were identified by MALDI-TOFMS and n-ESIQ(q)TOFMS as isoforms of haptoglobin-1 precursor (HAP1)

  serum tryptic 2de-separated paraffin-embedded two-dimensional affi-gel maldi-tofms

• Genomic and proteomic profiling I: leiomyomas in African Americans and Caucasians.

  PMID 17716379 · PMC2063502 · Reproductive biology and endocrinology : RB&E · 2007 · 7 claims · 6 setups

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  268 genes (177 over-expressed, 91 under-expressed) are differentially expressed in leiomyomas of African Americans compared to Caucasians (P<0.01, 2-fold cutoff)

  paired same excised paraffin-embedded microarray realtime 2d-page

• Plasma proteomics of pancreatic cancer patients by multi-dimensional liquid chromatography and two-dimensional difference gel electrophoresis (2D-DIGE): up-regulation of leucine-rich alpha-2-glycoprotein in pancreatic cancer.

  PMID 17303479 · PMC7105233 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2007 · 5 claims · 5 setups

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  Pre-fractionation of plasma with immuno-affinity depletion and anion-exchange chromatography prior to 2D-DIGE substantially increases the number of detectable protein spots compared with unfractionated plasma.

  plasma excised fractionated 2d-dige multi-dimensional lc-ms