Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

• A quantitative proteomics analysis of subcellular proteome localization and changes induced by DNA damage.

  PMID 20026476 · PMC2849709 · Molecular & cellular proteomics : MCP · 2010 · 6 claims · 5 setups

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  A SILAC-based 'spatial proteomics' method can quantitatively measure the relative subcellular distribution of thousands of proteins across cytoplasm, nucleus, and nucleolus.

  hct116 silac silac-based immunofluorescence

• A novel approach to tag and identify geranylgeranylated proteins.

  PMID 19784953 · PMC2855049 · Electrophoresis · 2009 · 7 claims · 7 setups

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  Metabolic incorporation of azido-GG alcohol followed by Cu(I)-catalyzed click reaction with TAMRA-alkyne selectively labels geranylgeranylated proteins for fluorescence detection

  jurkat cos-7 mcf-7 embryonic metabolic comparative fractionation

• Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein.

  PMID 16507314 · PMC7111751 · Virology · 2006 · 7 claims · 8 setups

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  Expression of SARS-CoV E protein in mammalian cells alters membrane permeability

  hela sf9 hygromycin site-directed subcellular

• Metabolism as a complex genetic trait, a systems biology approach: implications for inborn errors of metabolism and clinical diseases.

  PMID 18836848 · PMC4319114 · Journal of inherited metabolic disease · 2008 · 7 claims · 8 setups

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  Synergistic heterozygosity — cumulative heterozygous mutations at multiple loci in functionally related metabolic pathways — can cause physiologically relevant reduction of pathway flux and disease.

  models liver sucrose vlcad-deficient physiological blue sucrose

• Bladder tumour-derived somatic TSC1 missense mutations cause loss of function via distinct mechanisms.

  PMID 18397877 · PMC2427143 · Human molecular genetics · 2008 · 8 claims · 8 setups

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  All six somatic TSC1 missense mutations found in bladder tumours cause loss of TSC1 function, but via distinct molecular mechanisms.

  hcv29 lines tsc1 bladder immunoblotting real-time co-immunoprecipitation

• TFEB deficiency attenuates mitochondrial degradation upon brown adipose tissue whitening at thermoneutrality.

  PMID 33516944 · PMC7903014 · Molecular metabolism · 2021 · 7 claims · 8 setups

  Has reproduction

  BAT whitening at thermoneutrality is associated with TFEB-dependent mitochondrial degradation.

  bat subscapular tfeb-bat-ko metabolically qrt-pcr western immunohistochemistry

• What defines a photosynthetic microbial mat in western Antarctica?

  PMID 40043057 · PMC11882083 · PloS one · 2025 · 6 claims · 8 setups

  Has reproduction · 85

  Taxonomic composition of Antarctic microbial mat communities is characterized by similar bacterial groups across regions

  microbial quality-filtered overflowing shotgun taxonomic alpha

• Pathway analysis of kidney cancer using proteomics and metabolic profiling.

  PMID 17123452 · PMC1665458 · Molecular cancer · 2006 · 8 claims · 8 setups

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  31 proteins are differentially expressed with high statistical significance (p<0.05) in ccRCC tumor tissue compared to adjacent non-malignant kidney tissue

  ccrcc rcc computational random gel immunoblotting immunohistochemistry

• Solute exchange through gap junctions lessens the adverse effects of inactivating mutations in metabolite-handling genes.

  PMID 36107487 · PMC9534548 · eLife · 2022 · 8 claims · 8 setups

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  Colorectal cancer (CRC) cells are coupled by gap junctions assembled predominantly from Cx26 (GJB2)

  colorectal normal crc microarray tcga single-cell

• Cancer-cell-secreted miR-204-5p induces leptin signalling pathway in white adipose tissue to promote cancer-associated cachexia.

  PMID 37620316 · PMC10449837 · Nature communications · 2023 · 8 claims · 8 setups

  Has reproduction · 85

  Breast cancer cell-secreted exosomal miR-204-5p induces HIF1A in white adipose tissue by targeting the VHL gene.

  balb nsg ewat iwat orthotopic indirect sev

• Tumor cell metabolism: the marriage of molecular genetics and proteomics with cellular intermediary metabolism; proceed with caution!

  PMID 17090311 · PMC1636067 · Molecular cancer · 2006 · 8 claims · 5 setups

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  Genetic/proteomic alterations will have little relevancy to disease processes if they are not manifested in altered and impaired cellular and metabolic function

  prostate tumor malignant enzyme gene abundance

• SECIS elements in the coding regions of selenoprotein transcripts are functional in higher eukaryotes.

  PMID 17169995 · PMC1802603 · Nucleic acids research · 2007 · 8 claims · 5 setups

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  SECIS elements located within coding regions of selenoprotein mRNAs support functional Sec insertion in mammalian cells

  viral hek293 hepatoma tblastn computational western

• Proteomic analysis of ovarian cancer cells reveals dynamic processes of protein secretion and shedding of extra-cellular domains.

  PMID 18560578 · PMC2409963 · PloS one · 2008 · 8 claims · 6 setups

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  Ovarian cancer cells exhibit extensive shedding of extra-cellular domains from cell surface proteins into the extracellular milieu.

  ovcar3 analysis lc-ms cell-surface silac

• Modified H5 promoter improves stability of insert genes while maintaining immunogenicity during extended passage of genetically engineered MVA vaccines.

  PMID 19969118 · PMC2821965 · Vaccine · 2010 · 7 claims · 8 setups

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  rMVA expressing multiple antigens under the mH5 promoter exhibit remarkable genetic stability and maintain potent immunogenicity after serial passage

  rmva-infected individually bhk-21 pbmc qpcr western southern

• 5-HT reuptake blockade induces pyroptosis in BRAF(V600E)-mutated melanomas via remodeling histone serotonylation.

  PMID 41494533 · PMC12866116 · Cell reports. Medicine · 2026 · 8 claims · 8 setups

  Has reproduction · 40

  High-throughput screening of 90 FDA-approved antidepressants identifies PH as an alternative therapeutic for BRAF V600E-mutated melanoma

  braf a375 gfp-tph1-overexpressing yumm1.7 high-throughput ic50 xenograft

• Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteins.

  PMID 17446074 · PMC1885954 · Current biology : CB · 2007 · 8 claims · 8 setups

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  Newly synthesized ribosomal proteins accumulate in nucleoli more quickly than other nucleolar proteins

  hela post-mitotic pulsed fluorescence selective

• Proteomic approaches to cancer biomarkers.

  PMID 19931265 · PMC2873613 · Gastroenterology · 2010 · 8 claims · 8 setups

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  Combining abundant-protein depletion, offline fractionation, and subproteome (e.g., glycoproteome) enrichment with 2D LC-MS/MS increases the dynamic range and depth of blood proteome analysis for biomarker discovery.

  plasma serum capan-2 apcδ580 lectin silac elisa

• Intact innervation is essential for diet-induced recruitment of brown adipose tissue.

  PMID 30576247 · PMC6459298 · American journal of physiology. Endocrinology and metabolism · 2019 · 8 claims · 6 setups

  Has reproduction

  Intact innervation of BAT is essential for diet-induced thermogenesis/recruitment.

  interscapular whole-body adipose ibat surgical indirect western

• Genome-wide DNA hypermethylation opposes healing in patients with chronic wounds by impairing epithelial-mesenchymal transition.

  PMID 35819852 · PMC9433101 · The Journal of clinical investigation · 2022 · 8 claims · 8 setups

  Has reproduction · 68

  Chronic wound-edge tissue exhibits genome-wide DNA hypermethylation, with hypermethylation more prominent than hypomethylation across differentially methylated regions.

  chronic unwounded keratinocytes whole-genome bisulfite bulk

• Heterogeneity and Differentiation Trajectories of Infiltrating CD8+ T Cells in Lung Adenocarcinoma.

  PMID 36358600 · PMC9658355 · Cancers · 2022 · 7 claims · 8 setups

  Has reproduction · 67

  Infiltrating CD8+ T cells in LUAD can be divided into ten transcriptionally distinct subsets: eight cytotoxic (CTL) subsets, one naive-like (NTL) subset, and one exhausted (ETL) subset.

  luad tcga cd8+ degs single-cell cibersort survival