Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

Publications 1.3k Datasets 392.5k Experiments 132

System / cell line: hela ✕ Clear

Melusin gene (ITGB1BP2) nucleotide variations study in hypertensive and cardiopathic patients.

PMID 20017903 · PMC2803168 · BMC medical genetics · 2009 · 6 claims · 5 setups

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Only three nucleotide variations in ITGB1BP2 were found among 928 screened subjects, indicating a high degree of conservation of the gene in the populations analyzed

genomic hela itgb1bp2 melusin direct minigene in-silico
Proteomic identification of heterogeneous nuclear ribonucleoprotein L as a novel component of SLM/Sam68 Nuclear Bodies.

PMID 19912651 · PMC2784748 · BMC cell biology · 2009 · 7 claims · 7 setups

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hnRNP L is a novel Sam68-interacting protein partner identified by proteomics and confirmed by co-immunoprecipitation

lncap hek293 nih3t3 co-immunoprecipitation sucrose indirect
A dual pressure linear ion trap Orbitrap instrument with very high sequencing speed.

PMID 19828875 · PMC2816009 · Molecular & cellular proteomics : MCP · 2009 · 7 claims · 6 setups

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A stacked-ring ion guide (S-lens) increases ion transmission from the source into the instrument roughly 10-fold in MS/MS mode and 3-5-fold in full scan mode

intact bsa hela model top-down nanoflow ion
Connecting synthetic chemistry decisions to cell and genome biology using small-molecule phenotypic profiling.

PMID 19825513 · PMC2787914 · Current opinion in chemical biology · 2009 · 8 claims · 8 setups

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Multidimensional phenotypic profiling leverages information content from multiple parallel or multiplexed measurements of compound action on cells, unlike hierarchical screening which filters to few 'interesting' compounds.

one nci60 cerevisiae cancer multidimensional cellular two-color
Effects of two common polymorphisms in the 3' untranslated regions of estrogen receptor beta on mRNA stability and translatability.

PMID 19754929 · PMC2759954 · BMC genetics · 2009 · 8 claims · 4 setups

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Breast tumor heterozygotes show a significant difference in relative mRNA levels between the two alleles of rs4986938

breast hek293 erβ1 allelic luciferase stability
No direct effect of the -521 C/T polymorphism in the human dopamine D4 receptor gene promoter on transcriptional activity.

PMID 16723017 · PMC1481588 · BMC molecular biology · 2006 · 8 claims · 3 setups

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The -521 C/T SNP has no significant effect on transcriptional activity of the DRD4 promoter in any of three neural cell lines tested

sk-n-f1 rt-pcr luciferase
Mathematical models in mammalian cell biology.

PMID 18638360 · PMC2530880 · Genome biology · 2008 · 8 claims · 8 setups

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A point mutation in the circadian Per2 gene causes familial advanced sleep phase syndrome (FASPS) via a phosphorylation defect that alters PER2 stability and subcellular localization.

culture skin hela mammalian live-cell confocal phosphoprotein
GenomeRNAi: a database for cell-based RNAi phenotypes. 2009 update.

PMID 19910367 · PMC2808900 · Nucleic acids research · 2010 · 7 claims · 7 setups

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GenomeRNAi is a database integrating RNAi phenotypes, reagents, and gene annotations from cell-based screens in Drosophila and human cells

drosophila hela genome-scale image-based rna-seq
miRGen 2.0: a database of microRNA genomic information and regulation.

PMID 19850714 · PMC2808909 · Nucleic acids research · 2010 · 7 claims · 6 setups

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miRGen 2.0 is a database providing comprehensive information about the genomic position of human and mouse microRNA coding transcripts and their regulation by transcription factors

lung organ malme embryonic polii small nucleosome
TGF-β-mediated activation of fibroblasts in cervical cancer: implications for tumor microenvironment and prognosis.

PMID 40124621 · PMC11929507 · PeerJ · 2025 · 8 claims · 8 setups

Has reproduction · 79

TGF-β signaling activity is significantly increased in cervical cancer fibroblasts compared to normal fibroblasts and promotes their proliferation/differentiation.

cervical fibroblasts tcga-cesc hela single-cell pseudo-time cell-cell
Discovery and functional interrogation of SARS-CoV-2 RNA-host protein interactions.

PMID 33743211 · PMC7951565 · Cell · 2021 · 8 claims · 6 setups

Has reproduction · 84

ChIRP-MS identifies 309 host proteins that bind SARS-CoV-2 RNA during active infection across Huh7.5 and Vero E6 cells.

huh7.5 host lung chirp-ms chirp-rna-seq sds-page
A missense mutation (Q279R) in the fumarylacetoacetate hydrolase gene, responsible for hereditary tyrosinemia, acts as a splicing mutation.

PMID 11476670 · PMC35353 · BMC genetics · 2001 · 8 claims · 7 setups

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The Q279R missense mutation acts as a splicing mutation in vivo, causing skipping of exon 9 (alone or with exon 8) rather than simply altering the encoded amino acid.

microdissected resected non-tumor liver pcr-rflp immunohistochemistry western
Proteomic analysis of the mammalian nuclear pore complex.

PMID 12196509 · PMC2173148 · The Journal of cell biology · 2002 · 8 claims · 7 setups

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Mass spectrometry was used to identify all proteins present in a biochemically purified rat liver NPC fraction.

liver empigen transiently mass sds-page electron
Are p.I148T, p.R74W and p.D1270N cystic fibrosis causing mutations?

PMID 15287992 · PMC509248 · BMC medical genetics · 2004 · 8 claims · 7 setups

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A CF-causing second mutation (c.3199del6 or the novel c.3395insA) was found in cis with p.I148T in all CF patients in this cohort who carried p.I148T

genomic family chromosomes hela dgge oligonucleotide restriction
A combined approach exploring gene function based on worm-human orthology.

PMID 15877817 · PMC1112593 · BMC genomics · 2005 · 8 claims · 6 setups

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Strict phylogenetic criteria (concordant Neighbor Joining and Maximum Parsimony tree topology) can select single most-likely human orthologs for C. elegans genes despite the large phylogenetic distance between worm and human sequences.

sapiens elegans hela phylogenetic rnai gfp
Identification of functional SNPs in the 5-prime flanking sequences of human genes.

PMID 15717931 · PMC550646 · BMC genomics · 2005 · 6 claims · 5 setups

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7 of 10 candidate SNPs tested by EMSA showed reproducible allele-specific differences in TF-DNA complex binding/stability

flanking synthetic genomic cloned comparative electrophoretic rflp
Diversity of tRNA genes in eukaryotes.

PMID 17088292 · PMC1693877 · Nucleic acids research · 2006 · 8 claims · 6 setups

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The number of tRNA genes having the same anticodon but different sequences elsewhere (isodecoder genes) varies significantly (10–246) across 11 eukaryotes despite isoacceptor numbers being similar (41–55)

eukaryotic bacterial genome synthetic genome-wide sequence manual
Lysine 63-polyubiquitination guards against translesion synthesis-induced mutations.

PMID 16789823 · PMC1513265 · PLoS genetics · 2006 · 8 claims · 8 setups

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K63-polyubiquitin chain formation protects human cells against translesion synthesis-induced mutations by promoting error-free recovery of blocked replication forks.

a549 western proliferation clonogenic
Mutations in pericentrin cause Seckel syndrome with defective ATR-dependent DNA damage signaling.

PMID 18157127 · PMC2397541 · Nature genetics · 2008 · 8 claims · 8 setups

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Homozygous truncating mutations in PCNT cause Seckel syndrome

consanguineous genomic lymphoblastoid lcls snp-microarray sanger immunofluorescence
Alternative splicing of human peroxisome proliferator-activated receptor delta (PPAR delta): effects on translation efficiency and trans-activation ability.

PMID 17705821 · PMC2045109 · BMC molecular biology · 2007 · 8 claims · 8 setups

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Multiple alternatively spliced 5'-UTR isoforms of human PPARdelta mRNA differ in translation efficiency

placenta five lines huh7 5'-race rt-pcr real-time

Experiments

Melusin gene (ITGB1BP2) nucleotide variations study in hypertensive and cardiopathic patients.

Proteomic identification of heterogeneous nuclear ribonucleoprotein L as a novel component of SLM/Sam68 Nuclear Bodies.

A dual pressure linear ion trap Orbitrap instrument with very high sequencing speed.

Connecting synthetic chemistry decisions to cell and genome biology using small-molecule phenotypic profiling.

Effects of two common polymorphisms in the 3' untranslated regions of estrogen receptor beta on mRNA stability and translatability.

No direct effect of the -521 C/T polymorphism in the human dopamine D4 receptor gene promoter on transcriptional activity.

Mathematical models in mammalian cell biology.

GenomeRNAi: a database for cell-based RNAi phenotypes. 2009 update.

miRGen 2.0: a database of microRNA genomic information and regulation.

TGF-β-mediated activation of fibroblasts in cervical cancer: implications for tumor microenvironment and prognosis.

Discovery and functional interrogation of SARS-CoV-2 RNA-host protein interactions.

A missense mutation (Q279R) in the fumarylacetoacetate hydrolase gene, responsible for hereditary tyrosinemia, acts as a splicing mutation.

Proteomic analysis of the mammalian nuclear pore complex.

Are p.I148T, p.R74W and p.D1270N cystic fibrosis causing mutations?

A combined approach exploring gene function based on worm-human orthology.

Identification of functional SNPs in the 5-prime flanking sequences of human genes.

Diversity of tRNA genes in eukaryotes.

Lysine 63-polyubiquitination guards against translesion synthesis-induced mutations.

Mutations in pericentrin cause Seckel syndrome with defective ATR-dependent DNA damage signaling.

Alternative splicing of human peroxisome proliferator-activated receptor delta (PPAR delta): effects on translation efficiency and trans-activation ability.