Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

Publications 1.3k Datasets 392.5k Experiments 44

System / cell line: in-gel ✕ Clear

Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors.

PMID 16901338 · PMC1779605 · Genome biology · 2006 · 8 claims · 4 setups

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491 proteins were identified in human tear fluid using in-gel digestion and LC-MS/MS/MS3 on LTQ-FT and LTQ-Orbitrap instruments

closed-eye tear analysis sds-page in-solution gene
Sister DNA Entrapment between Juxtaposed Smc Heads and Kleisin of the Cohesin Complex.

PMID 31201089 · PMC6675936 · Molecular cell · 2019 · 8 claims · 5 setups

Has reproduction · 64

Smc1 and Smc3 ATPase heads adopt two distinct in vivo states: ATP-dependent engaged (E) and signature-motif juxtaposed (J).

cerevisiae cysteine dual-interface cross-linking
Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.

PMID 18683930 · PMC2649877 · Journal of the American Chemical Society · 2008 · 7 claims · 5 setups

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Y289L GalT-mediated transfer of UDP-GalNAz followed by Cu(I)-catalyzed azide-alkyne cycloaddition enables selective, highly sensitive fluorescent/biotin labeling of O-GlcNAc-modified proteins

α-crystallin forebrain hela chemoenzymatic in-gel nanolc-ms
In vivo pharmaco-proteomic analysis of hydroxyurea induced changes in the sickle red blood cell membrane proteome.

PMID 19914412 · PMC2818491 · Journal of proteomics · 2010 · 8 claims · 4 setups

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2D-DIGE combined with tandem mass spectrometry identified 32 distinct sickle RBC membrane proteins that significantly changed in abundance after in vivo HU therapy

sickle excised patients 2d-dige tandem immunoblot
Preclinical and post-treatment changes in the HCC-associated serum proteome.

PMID 17060939 · PMC2360589 · British journal of cancer · 2006 · 7 claims · 6 setups

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SELDI proteomic profiling of serum reveals consistent, statistically significant changes associated with the development of HCC in hepatitis C patients

serum pooled excised seldi-tof anion lc-ms
The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins.

PMID 16948836 · PMC1794545 · Genome biology · 2006 · 8 claims · 6 setups

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Identified 1543 proteins in urine from ten healthy donors while essentially eliminating false-positive identifications

urine pooled urinary identified sds-page albumin decoy
Proteomic identification of nitrated brain proteins in amnestic mild cognitive impairment: a regional study.

PMID 17760844 · PMC3823261 · Journal of cellular and molecular medicine · 2007 · 8 claims · 5 setups

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Total protein nitration (3-NT immunoreactivity) is significantly increased in MCI hippocampus and IPL compared to age-matched control brain

postmortem excised mci 2d-page sypro in-gel
Comparative cytochrome P450 proteomics in the livers of immunodeficient mice using 18O stable isotope labeling.

PMID 17296599 · PMC2315784 · Molecular & cellular proteomics : MCP · 2007 · 8 claims · 5 setups

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SDS-PAGE combined with post-digest 18O/16O labeling and LC-MS/MS enables relative quantification of multiple P450 proteins from liver microsomes, including highly homologous isoforms

liver recombinant tryptic sds-page western mascot
Proteomic analysis of protein deposits on worn daily wear silicone hydrogel contact lenses.

PMID 18989384 · PMC2579937 · Molecular vision · 2008 · 8 claims · 4 setups

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Worn silicone hydrogel contact lenses deposit a wide array of proteins from the tear film and other sources.

deposit excised pooled worn sds-page in-gel lc-ms-ms
Pharmaco-proteomic study of hydroxyurea-induced modifications in the sickle red blood cell membrane proteome.

PMID 18849548 · PMC4260454 · Experimental biology and medicine (Maywood, N.J.) · 2008 · 6 claims · 6 setups

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50 μM HU treatment of SS RBC membranes causes statistically significant changes in a discrete set of membrane proteins, distinct from HbF induction.

sickle rbc excised 2d-dige tandem immunoblot
Investigation of the human tear film proteome using multiple proteomic approaches.

PMID 18334958 · PMC2268847 · Molecular vision · 2008 · 8 claims · 6 setups

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Tear collection method (capillary vs. Schirmer strip) significantly impacts which proteins are detected in the tear film proteome.

tear spectra 1d-sds-page 2d-sds-page mudpit
Immunohistochemical and proteomic evaluation of nuclear ubiquitous casein and cyclin-dependent kinases substrate in invasive ductal carcinoma of the breast.

PMID 20069058 · PMC2801467 · Journal of biomedicine & biotechnology · 2009 · 7 claims · 5 setups

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NUCKS is highly overexpressed in invasive ductal carcinoma (IDC) of the breast compared to matched normal tissue.

matched idc nucks rabbits lc-ms sds-page western
Proteomic and phospho-proteomic profile of human platelets in basal, resting state: insights into integrin signaling.

PMID 19859549 · PMC2762604 · PloS one · 2009 · 8 claims · 8 setups

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A comprehensive platelet proteome of 1507 unique proteins was identified from ten independent human platelet samples, the most comprehensive platelet proteome assembled to date

platelets platelet anti-coagulated lc-ms phospho-proteomic membrane
Use of proteomics for validation of the isolation process of clotting factor IX from human plasma.

PMID 19819359 · PMC2818390 · Journal of proteomics · 2010 · 8 claims · 8 setups

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Proteomic techniques (LC-MS/MS, iTRAQ, immunochemical/biochemical methods) can monitor and validate each production step of pd F IX isolation

plasma in-process chromatographic one-stage competitive elisa
Proteomic analysis of serum in lung cancer induced by 3-methylcholanthrene.

PMID 19794824 · PMC2753780 · Journal of biomedicine & biotechnology · 2009 · 7 claims · 4 setups

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141 protein spots are differentially expressed between normal rat sera and MCA-induced lung cancer rat sera (82 overexpressed, 59 underexpressed)

serum lung excised two-dimensional maldi-tof-ms histopathology
The use of neuroproteomics in drug abuse research.

PMID 19926406 · PMC3947580 · Drug and alcohol dependence · 2010 · 8 claims · 8 setups

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Neuroproteomic technologies (2D-DIGE, iTRAQ, ICAT, etc.) enable identification of protein-level changes underlying effects of drugs of abuse such as amphetamine, morphine, cocaine, and alcohol.

rodent brain postmortem sectioned 2d-dige itraq heat
MAPU: Max-Planck Unified database of organellar, cellular, tissue and body fluid proteomes.

PMID 17090601 · PMC1781136 · Nucleic acids research · 2007 · 8 claims · 8 setups

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MAPU is a unified, freely accessible database integrating organellar, cellular, tissue and body fluid proteomes generated by high-resolution mass spectrometry.

urine tear seminal breast gel lc-ms3 in-depth
Identification of novel citrullinated autoantigens of synovium in rheumatoid arthritis using a proteomic approach.

PMID 17125526 · PMC1794520 · Arthritis research & therapy · 2006 · 8 claims · 6 setups

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51 citrullinated protein spots were detected in RA synovial tissue, of which 30 (58.8%) were autoantigenic (reactive with RA sera)

synovial excised coli recombinant two-dimensional maldi-tof recombinant
Large-scale and high-confidence proteomic analysis of human seminal plasma.

PMID 16709260 · PMC1779515 · Genome biology · 2006 · 8 claims · 6 setups

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923 proteins were identified with high confidence in seminal plasma from a single individual, combining results from three ejaculate samples

seminal same list combined sds-page database decoy
Proteomic analysis of nipple aspirate fluid from women with early-stage breast cancer using isotope-coded affinity tags and tandem mass spectrometry reveals differential expression of vitamin D binding protein.

PMID 16542425 · PMC1431555 · BMC cancer · 2006 · 8 claims · 5 setups

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ICAT tandem MS can identify and quantify differences in specific protein expression between NAF from tumor-bearing and disease-free breasts

paired icat-labeled spectra naf icat sds-page liquid

Experiments

Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors.

Sister DNA Entrapment between Juxtaposed Smc Heads and Kleisin of the Cohesin Complex.

Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.

In vivo pharmaco-proteomic analysis of hydroxyurea induced changes in the sickle red blood cell membrane proteome.

Preclinical and post-treatment changes in the HCC-associated serum proteome.

The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins.

Proteomic identification of nitrated brain proteins in amnestic mild cognitive impairment: a regional study.

Comparative cytochrome P450 proteomics in the livers of immunodeficient mice using 18O stable isotope labeling.

Proteomic analysis of protein deposits on worn daily wear silicone hydrogel contact lenses.

Pharmaco-proteomic study of hydroxyurea-induced modifications in the sickle red blood cell membrane proteome.

Investigation of the human tear film proteome using multiple proteomic approaches.

Immunohistochemical and proteomic evaluation of nuclear ubiquitous casein and cyclin-dependent kinases substrate in invasive ductal carcinoma of the breast.

Proteomic and phospho-proteomic profile of human platelets in basal, resting state: insights into integrin signaling.

Use of proteomics for validation of the isolation process of clotting factor IX from human plasma.

Proteomic analysis of serum in lung cancer induced by 3-methylcholanthrene.

The use of neuroproteomics in drug abuse research.

MAPU: Max-Planck Unified database of organellar, cellular, tissue and body fluid proteomes.

Identification of novel citrullinated autoantigens of synovium in rheumatoid arthritis using a proteomic approach.

Large-scale and high-confidence proteomic analysis of human seminal plasma.

Proteomic analysis of nipple aspirate fluid from women with early-stage breast cancer using isotope-coded affinity tags and tandem mass spectrometry reveals differential expression of vitamin D binding protein.