Experiments

Searchable full-text extractions: founding hypothesis, core claims, experimental setups, key results and statistics — pulled out of each paper as structure. Search a cell line, an assay or an entity (e.g. HUH7) and find every paper that worked with it. This corpus stands on its own: most entries carry no reproduction assessment (yet).

• Characterization of a natural mutator variant of human DNA polymerase lambda which promotes chromosomal instability by compromising NHEJ.

  PMID 19806195 · PMC2751832 · PloS one · 2009 · 8 claims · 8 setups

  Full-text index only

  The W438 hPolλ variant has reduced base substitution fidelity in vitro compared to wild-type R438

  normal recombinant hpolλ short pcr-sscp ber m13mp2

• Dissection of multiple sclerosis genetics identifies B and CD4+ T cells as driver cell subsets.

  PMID 35672799 · PMC9175345 · Genome biology · 2022 · 8 claims · 6 setups

  Has reproduction

  CD4 T cells and B cells independently mediate MS GWAS genetic signals through their open chromatin regions, beyond shared regulatory landscapes.

  flow-sorted cd4 gwas bulk ldsc

• Loss of CD4(+) T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection.

  PMID 37572656 · PMC10576612 · Immunity · 2023 · 8 claims · 8 setups

  Has reproduction · 68

  Arg1 expression is induced in lung CD4+ T cells during in vivo influenza infection, ranking among the most highly induced genes.

  flow-sorted cd3 lung cd4+ bulk flow western

• Spatial transcriptomics demonstrates the role of CD4 T cells in effector CD8 T cell differentiation during chronic viral infection.

  PMID 36450262 · PMC9792173 · Cell reports · 2022 · 7 claims · 7 setups

  Has reproduction · 50

  Following transient CD4 depletion, IL-21-producing Tfh cells do repopulate but are outnumbered by immunomodulatory CD4 T cells (Tregs, Th17), and IL-21+ frequency remains low within activated CD4 T cells.

  il-21-trfp cd44+ splenocytes chronically flow scrna-seq adoptive

• JunB Is Critical for Survival of T Helper Cells.

  PMID 35837408 · PMC9273772 · Frontiers in immunology · 2022 · 7 claims · 7 setups

  Has reproduction · 69

  JunB is required for clonal expansion of Th1, Th2, and Th17 cells

  ot-ii junb naive th1 flow adoptive th1

• Competitive binding of STATs to receptor phospho-Tyr motifs accounts for altered cytokine responses.

  PMID 33871355 · PMC8099432 · eLife · 2021 · 8 claims · 8 setups

  Has reproduction · 89

  IL-27 induces more sustained and higher-amplitude STAT1 phosphorylation than HypIL-6, while both induce comparable STAT3 phosphorylation

  th-1 rpe1 t-cells expressing high-throughput dual-color single-molecule

• Intergenic risk variant rs56258221 skews the fate of naive CD4(+) T cells via miR4464-BACH2 interplay in primary sclerosing cholangitis.

  PMID 38901430 · PMC11293351 · Cell reports. Medicine · 2024 · 8 claims · 8 setups

  Has reproduction · 58

  rs56258221 (BACH2/MIR4464) associates with a distinct peripheral blood T cell immunophenotype in people with PSC

  peripheral liver facs-sorted cd4+ flow bulk cite-seq

• Antigen and checkpoint receptor engagement recalibrates T cell receptor signal strength.

  PMID 34534438 · PMC8585507 · Immunity · 2021 · 6 claims · 7 setups

  Has reproduction · 37

  TCR signal strength drives dynamic, dose- and time-dependent transcriptional changes in CD4+ T cells while single-cell Nr4a3 activation dynamics remain digital/uniform.

  tg4 sorted live melanoma flow rna-seq re-stimulation

• Depletion of HIV reservoir by activation of ISR signaling in resting CD4(+)T cells.

  PMID 36590168 · PMC9800255 · iScience · 2023 · 8 claims · 8 setups

  Has reproduction · 89

  Activation of ISR/ATF4 signaling (via HA15) reverses HIV latency in Jurkat models of HIV latency (J-Lat A1, 2D10)

  jurkat cd4+ hiv-negative resting flow trypan western

• Development of double-positive thymocytes at single-cell resolution.

  PMID 33771202 · PMC8004397 · Genome medicine · 2021 · 7 claims · 8 setups

  Has reproduction · 86

  DP thymocytes can be classified into blast, rearrangement, and selection subtypes, distinguishable by surface markers CD2 and Ly6d

  thymocytes sorted thymocyte scrna-seq flow immunofluorescence

• Blood and tissue correlates of steroid non-response in checkpoint inhibition-induced immune-related adverse events.

  PMID 41254329 · PMC12627558 · Communications medicine · 2025 · 7 claims · 6 setups

  Has reproduction · 71

  An enhanced type 1/type 17 (Th1/Th17, TC1/TC17) immune response in blood and tissue is associated with steroid non-response in irAEs

  pbmcs pre-steroids extended snap-frozen spectral pd-1 ici-bound

• Vaccine-induced ICOS(+)CD38(+) circulating Tfh are sensitive biosensors of age-related changes in inflammatory pathways.

  PMID 34095875 · PMC8149371 · Cell reports. Medicine · 2021 · 8 claims · 5 setups

  Has reproduction · 88

  Vaccine-induced ICOS+CD38+ cTfh from elderly adults show enriched TNF-NF-κB signaling compared to young adults at day 7 post-vaccination

  pbmc facs-sorted icos+cd38+ autologous flow bulk ctfh-b

• Single-cell RNA-sequencing highlights a curtailed NK cell function in convalescent COVID-19 pregnant women.

  PMID 40661959 · PMC12257036 · Frontiers in immunology · 2025 · 7 claims · 5 setups

  Has reproduction · 87

  NK cells show a sustained reduction during active SARS-CoV-2 infection and persisting after recovery in pregnant women

  pbmcs serum 14-color single-cell 13-plex

• De novo identification of CD4(+) T cell epitopes.

  PMID 38658646 · PMC11093748 · Nature methods · 2024 · 7 claims · 8 setups

  Has reproduction · 83

  SABR-IIs (chimeric receptors linking a covalently attached peptide-MHC-II to CD28-CD3ζ signaling domains) present epitopes to CD4+ T cells and induce a readable NFAT-GFP/CD69 signal upon cognate TCR recognition

  nfat-gfp hla-dq8 5kc daudi nfat-gfp co-incubation species

• comBO: A combined human bone and lympho-myeloid bone marrow organoid for preclinical modeling of hematopoietic disorders.

  PMID 41734765 · PMC7618947 · Cell stem cell · 2026 · 8 claims · 8 setups

  Has reproduction · 65

  comBO is a single iPSC differentiation generating lymphoid, myeloid, vascular, mesenchymal stromal, and functional osteo/adipogenic lineages within one organoid

  ipsc-derived combo combo-derived mscarlet-tagged flow single-cell confocal

• Severe combined immunodeficiency (SCID) and attention deficit hyperactivity disorder (ADHD) associated with a Coronin-1A mutation and a chromosome 16p11.2 deletion.

  PMID 19097825 · PMC2692687 · Clinical immunology (Orlando, Fla.) · 2009 · 8 claims · 7 setups

  Full-text index only

  CORO1A deficiency, caused by a paternal 2bp frameshift mutation combined with a maternal de novo 600kb deletion at chromosome 16p11.2, is the molecular cause of this patient's T-B+NK+ SCID

  ebv-transformed genomic father peripheral intracellular snp sequencing

• HIT: a versatile proteomics platform for multianalyte phenotyping of cytokines, intracellular proteins and surface molecules.

  PMID 18849997 · PMC3334282 · Nature medicine · 2008 · 8 claims · 8 setups

  Full-text index only

  HIT uses oligonucleotide-tagged (Fab- or mSA-conjugated) antibodies, T7 polymerase amplification, and DNA microarray hybridization to indirectly measure multiple analytes in a fluid-phase multiplex format

  hsp70-coated hsp70 secreted jurkat fluid-phase multiplex hit

• DeepRNA-Reg: a deep-learning based approach for comparative analysis of CLIP experiments.

  PMID 41055236 · PMC12505516 · RNA biology · 2025 · 7 claims · 5 setups

  Has reproduction · 100

  DeepRNA-Reg, a recurrent neural network-based algorithm, predicts differentially enriched sites in paired HITS-CLIP datasets and outperforms dCLIP 1.7.

  dgcr8 mir-23 deeprna-reg ago2 gene flow

• Functional diversity of HIV-1 envelope proteins expressed by contemporaneous plasma viruses.

  PMID 18312646 · PMC2270869 · Retrovirology · 2008 · 8 claims · 7 setups

  Full-text index only

  Infectivity of recombinant viruses carrying different Env proteins from the same patient varies over an approximately 10-fold range, even among viruses with similar tropism.

  env u373 mt4-r5 u373-r5 phylogenetic infectivity flow

• Liver-specific paraoxonase-1 alleviates regulatory T cell-driven immunosuppression via metabolic reprogramming in hepatocellular carcinoma.

  PMID 41387425 · PMC12701056 · Nature communications · 2025 · 8 claims · 8 setups

  Has reproduction · 66

  PON1 expression is downregulated in HCC tumor tissue and low PON1 is associated with worse prognosis

  hcc paired hepa1-6 pon1-nc bioinformatic immunohistochemistry orthotopic